Screening for inhibitors from medicinal plants extracted Against bacteria, fungi, GSK-3β and MKK1

Koay, Kean Huang (2007) Screening for inhibitors from medicinal plants extracted Against bacteria, fungi, GSK-3β and MKK1. University Malaysia Sabah. (Unpublished)

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Abstract

The objective of this project was to extract secondary metabolites from medicinal plants and to screen for phytochemicals; alkaloids, saponin, cardiac glycoside, tannin and polyphenol in the samples. It was also in determining the inhibition potential of plants extraction towards serine/threonine protein kinases, especially GSK-3β and MKK 1, bacteria and fungal. Total of 10 medicinal plants which are Nephrolepis auriculata, Selaginella doederleinii, Dicranopteris linearis, Bauhinia purpurea, Lonicera japol1ica, Synediella nodiflora, Cycas revoluta, Strobilanthus crispus, Opuntia dillenii and Kyllinga brevifolia, have been collected from Orchid De Villa, Inanam, Sabah. Only methanol extract has been tested for phytochemical analysis. In alkaloid test, Lonicera japonica showed precipitation in Mayer, Wagner and Dragendroff test while Cycas revoluta showed slight cloudy only in Dragendroff test. Gelatin test and FeCI₃ test was performed in tannin and polyphenol test. Nephrolepis auriculata and Opuntia dillenii extracts showed presence of tannin while Dicranopteris linearis, Cycas revoluta and Kyllinga brevifolia extracts showed presence of polyphenol. Selaginella doederleinii, Bauhinia purpurea, Lonicera japol1ica, Synediella nodiflora, Cycas revoluta, Strobilanthus crispus, Opuntia dillenii and Kyllinga brevifolia showed the presence of cardiac glycoside. In saponin test, foam test was carried out, Nephrolepis auriculata, Selaginella doederleinii, Dicranopteris linearis, Bauhinia purpurea, Lonicera japol1ica, Synediella nodiflora and Kyllinga brevifolia extracts showed positive result; meanwhile Strobilanthus crispus and Opuntia dillenii showed negative result mean the presence of free acid after added Na₂SO₄. In bacteria screening system, only E. coli, Staphylococcus aureus and Bacillus subtilis showed inhibition activity; meanwhile there is no activity against Samonella typhi and Enterobacter aerogenes and also Candida krusei and Candida albicans. Yeast strains H10075 transformed with pKT10-gsk-3β and latter transformed with YEP24-MCK1 was used n GSK-3β screening system. This system is based on temperature sensitivity (25°C and 37°C), in which this transformed yeast strain are able to suppress temperature sensitivity phenotype. Results found that no GSK-3β potential inhibitors found from the samples. In MKK1 screening system, all methanol extracts showed potential inhibitory activity. This system utilizes mutant yeast MKK1 P³��which is under control of the strong GAL1p. Over expression of the mutant gene will cause cell growth arrest with the addition of galactose.

Item Type: Academic Exercise
Uncontrolled Keywords: medicinal plant, extraction, inhibition, mutant gene, cell growth, galactose
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 14 May 2014 05:21
Last Modified: 13 Oct 2017 03:50
URI: http://eprints.ums.edu.my/id/eprint/9003

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