Fermentation dynamics of seaweed silage and its potential as probiotic fish feed

Sangeetha Priya Anangdan, (2010) Fermentation dynamics of seaweed silage and its potential as probiotic fish feed. Masters thesis, Universiti Malaysia Sabah.


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Optimization of the probiotic microbes fortified fermentation dynamics of seaweed powder, Eucheuma denticulatum and Kappaphycus alvarezii, was investigated via microbiology and chemical analysis. A total of seven types of fermentation were carried out for each seaweed using a cocktail of four strains of Lactic Acid Bacteria; Lactobacillus casel; LactobacIllus plantarum, Lactobacillus fermentum & Lactobacillus sakei , yeast; Saccharomyces cerevisiae, and cellulase enzyme. Research was divided into three phases; Phase 1- small-scale fermentation (100ml), Phase II - up-scale fermentation (1 L) and Phase III - 3 L fermentation for seaweed silage biomass production and live rotifer feed assay. Bacteria count and pH profile were recorded for all fermentation. In phase I, chemical analysis were carried out for moisture, ash, lipid and protein. In phase II additional analysis such as fatty acids, amino acids, organic acid and carrageenan size, were carried out. In Phase I, fermentation of both seaweed using Lactobacillus casei as a single strain and fermentation using a mixture of all four strains showed promising result. Out of these four fermentation that were up-scaled in Phase II, fermentation of Eucheuma denticulatum using Lactobacillus casei showed the best result in terms of bacteria count where there was an increment of 275 % in the first three days. The pH profile showed a drop in from 5.3 to 3.5. Lipid content was 1.9 %, protein 18% moisture 97.5% and ash 33.4%. Organic acids analysis indicated that lactic oxalic acid, fumaric acid and maleic acids were produced during fermentation. Carrageenan size analysis showed that carrageenan size was reduced from a size of 800KD to 5KD. Fatty acid analysis revealed the production of saturated fatty acids, mono-unsaturated fatty acids and polyunsaturated fatty acids while amino acid analysis showed a surge in free amino acids in the form of glutamic acid and glycine. The anti-microbial assay showed a 50 % inhibition against twelve species of tested microbes. Biomass production of this fermentation from Phase III was used in the live rotifer feed assay, which saw an increment of 224 % in rotifer density when the rotifers were fed with an equal mixture of Nannocloropsis and probiotic fortified seaweed silage. Hence, seaweed silage produced via fermentation with probiotic (L. casel) is a very promising product that could help our fisheries to increase larvae production.

Item Type: Thesis (Masters)
Uncontrolled Keywords: fermentation dynamic, seaweed silage, probiotic fish seed, fatty acid, carrageenan size, biomass production
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: SCHOOL > Institute for Tropical Biology and Conservation
Date Deposited: 07 Jul 2014 04:20
Last Modified: 30 Oct 2017 02:04
URI: http://eprints.ums.edu.my/id/eprint/9283

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