Effect of Metroxylon sago polyphenol feeding on the free radical scavenging enzymes in hamster tissue

Permah @ Perumal Ramasamy, Permah @ Perumal and Laura , D. and Melissa, H. (2005) Effect of Metroxylon sago polyphenol feeding on the free radical scavenging enzymes in hamster tissue. In: IUBMB 50th Anniversary Symposium, 02-07Jun 2005 , Budapest, Hungary.

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Official URL: http://dx.doi.org/10.1111/j.1742-4658.2005.4739_10...

Abstract

The aqueous extract of Metroxylon sago (SAE), containing polyphenols, was investigated for its antioxidant as well as free radical scavenging activities, using various chemical as well as enzymatic assays. The toxicity of the extract was evaluated by the brine shrimp test and was found to be non-toxic. The extract was fractionated into eighteen fractions by column chromatography on Sephadex LH20. Further purification was achieved by C18 reversed phase High Performance Liquid Chromatography. Dietary studies were carried out using the Syrian hamsters, Mesocricetus auratus. The hamsters were divided into three dietary groups, with the first being fed on a basal diet, as negative controls, the second being fed on a diet supplemented with butylated hydroxy toluene (BHT), as positive control and the third group, the experimental, being fed on a diet supplemented with the sago aqueous extract (SAE), for a period of eight weeks continuously. At the end of each week, for the first four weeks and at the end of the eighth week, the hamsters were sacrificed in groups of three from each dietary group and the tissues from brain, lungs, liver and kidneys assessed for the free radical-scavenging enzymes, superoxide dismutase (SOD), catalase (CA) and glutathione peroxidase (GPX). All the three enzymes assayed showed that SAE was a more potent antioxidant than BHT. The polyphenolic compounds from the aqueous extract of Metroxylon sago were shown to effectively lower the endogenous levels of free radicals in all these hamster tissues thereby reducing the activities of all the free radical-scavenging enzymes assayed.

Item Type:Conference Paper (UNSPECIFIED)
Uncontrolled Keywords:Biochemistry & Molecular Biology
Subjects:Q Science > QP Physiology
Divisions:SCHOOL > School of Medicine
ID Code:990
Deposited By:IR Admin
Deposited On:14 Oct 2011 08:13
Last Modified:29 Dec 2014 16:24

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