Detection of carbamic pesticide using a butyrylcholinesterase-based biosensor

Rafidah Ismail (2014) Detection of carbamic pesticide using a butyrylcholinesterase-based biosensor. Universiti Malaysia Sabah. (Unpublished)

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The biosensor provided a new and alternative tool for pesticides analysis that is sensitive, fast and inexpensive compared to the conventional methods for determination of carbamate pesticide using amperometric butyryicholinesterase (BChE) biosensor. In this study, the objective is to immobilize butyrylcholinesterase enzyme (BChE) based on chitosan (CHIT) onto glassy carbon electrode (GCE) through cross-linking immobilization method. The BChE biosensor was fabricated by firstly depositing CHIT film on glassy carbon electrode (GCE) and then attaching BChE on CHIT film using glutaraldehyde as a cross-linking agent. CHIT was selected as BCHE immobilization matrix due to its excellent mechanical strength and biocompatibility. The methylene blue was used as a redox mediator that shuttle electron transfer in BChE biosensor. Optimal conditions were pH 7, scan rate of 0.03 V and 10 s for BChE/CHIT/GCEto determine peak response of carbaryl inhibition in 0.2 M PBS-0.1M KCI solution in the presence of 0.4 mM BTCI as substrate. The inhibition of carbaryl as one of carbamate compound that has high toxicity showed a decline in response of peak current BChE/CHIT/GCE by reducing the immobilized BChE activity and thiocholine products. The lowest detection limit of carbaryl on BChE/CHIT/GCEw as 10 x1011 ng/ml.

Item Type: Academic Exercise
Uncontrolled Keywords: Biosensor, Pesticides Butyrylcholinesterase Chitosan, Methylene blue
Subjects: T Technology > TP Chemical technology
Divisions: FACULTY > Faculty of Science and Natural Resources
Date Deposited: 23 Nov 2017 12:56
Last Modified: 23 Nov 2017 12:56

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