Nanosensor-based-formaldehyde detection in fish

Shafiquzzaman Siddiquee and Kamaruzaman Hj. Ampon and Sujjat Al-Azad and Noor Aini Bohari Nanosensor-based-formaldehyde detection in fish. (Unpublished)

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Formaldehyde is used a preservative in fish by fisherman to maintain its fresh-look and prevent microbial spoilage. It can be affected human health and categorised as group 1 carcinogen. So there is needed a simple, sensitive and rapid device for determination of formaldehyde. The development of electrochemical biosensor was constructed by depositing an ionic liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate; [EMIM][Otf]),nanoparticles (ZnONPs, CaO and AuNPs), and chitosan (0-irD onto a glassy carbon electrode (GCE). The enzyme required B- nicotinamide adenine dinucleotide (NAD+) as a cofactor which was reduced to NADH during enzymatic reaction. Formaldehyde Dehydrogenase (FDH) was immobilized through the entrapment technique and measured on the reduction of NAD+ under a potential of 0.4 V. The morphological characteristics of nanomaterials and nanocomposite membrane were observed under scanning electron microscopy (SEM). Methylene blue was used as a redox indicator to increase the electron transfer capacity and stable in both the redox reaction. The optimisation parameters were found scan rate 0.1 V/s, temperature of 25°C, pH 7.0, and response time of s second. The storage stability was done for three month. Under the optimal conditions, the FDH/AuNPs/[EMIM][Otf]/CHIT/GCE was showed wider linear range of different formaldehyde concentrations from 0.01 to 10 ppm, with the detection limit of 0.1 ppm. The FDH/AuNPs/[EMIM][Otf]/CHIT was found more sensitive than FDH/ZnONPs/[EMIM][Otf]/CHIT and FDH/CaONPs/[EMIM][Otf]/CHIT, respectively. The formaldehyde biosensor was used for the detection of formalin on-site in fish samples; Malabar Trevally ( Carangoides malabaricuSJ, Malabar Red Snapper {Lutjanus Malabaricus), Longtail Tuna {Thunnus Tonggolj, and Narrow Barred Spanish Mackerel (Scomberomorus commerson) which were found below the safety level (5 ppm). The developed biosensor is a simple, rapid, and low cost when compared to the existing techniques.

Item Type: Research Report
Uncontrolled Keywords: Formaldehyde , fish , aquaculture
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Depositing User: NORAINI LABUK -
Date Deposited: 08 Jan 2020 16:28
Last Modified: 08 Jan 2020 16:28

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