Random amplification polymorphic DNA-PCR (RAPD) analysis of Vib rio algin oly tic u s strains isolated from green mussels ( Perna viridis ) in Marudu Bay, Sabah

Munirah Mohamad and Charles Santhanaraju Vairappan and Muhd Danish-Daniel and A. W. M. Effendy Random amplification polymorphic DNA-PCR (RAPD) analysis of Vib rio algin oly tic u s strains isolated from green mussels ( Perna viridis ) in Marudu Bay, Sabah. Jurnal Teknologi, 77 (25). pp. 45-49. ISSN 2180–3722

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Abstract

Vibrio alginolyticus infection is common in aquatic species. The species has been isolated from infected green mussels (Perna viridis) cultivated at Marudu Bay, Sabah. The infection of V. alginolyticus was prevalent since 2009 and caused more than 60% mortality of the mollusc. This study was conducted on V. alginolyticus isolated from clinically infected P. viridis at cultivation area in Marudu Bay from 2013. Twenty isolates were randomly selected and subjected to RAPD-PCR analysis using 20 sets of RAPD primers. Results showed that the most suitable primers were OPA 3, OPA 5, OPA 9, OPA 10 and OPA 12 where the significant bands ranging from 100-2500 bp. Genetic variability among the isolates as revealed by these primers indicated that RAPD-PCR method has good discriminative ability and can be used as a rapid typing method of differentiating V. alginolyticus strains isolated from infected green mussels for epidemiological investigation.

Item Type: Article
Uncontrolled Keywords: Vibrio alginolyticus, green mussels (Perna viridis), random amplified polymorphic DNA (RAPD)
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: INSTITUTE > Institute for Tropical Biology and Conservation
Depositing User: SITI AZIZAH BINTI IDRIS -
Date Deposited: 01 Jul 2020 11:15
Last Modified: 01 Jul 2020 11:15
URI: http://eprints.ums.edu.my/id/eprint/25560

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