First report of plant fungal pathogen Zasmidium musae associated with moribund eggs of ornate spiny lobster (Panulirus ornatus) in Sabah

Sin, Yin Yap and Katsuyuki Hamasaki and Balu Alagar Venmathi Maran and Audrey Daning Tuzan and Choon, Looi Ch’ng and Tamrin M. Lal (2020) First report of plant fungal pathogen Zasmidium musae associated with moribund eggs of ornate spiny lobster (Panulirus ornatus) in Sabah. [Experiment]

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Abstract

Fungal infections have been reported as one of the main hindrances for successful crustacean seed production. In a shrimp hatchery of Universiti Malaysia Sabah, abnormal sponge development of the ornate spiny lobster Panulirus ornatus was observed and it was suspected as fungal infection due to a change in sponge colour. Fungus was isolated from egg samples of P. ornatus and transferred to Peptone-Yeast-Glucose-Seawater (PYGS) agar to identify and to reveal its morphological characteristics. Interestingly, the isolate in PYGS broth transferred into sterilized seawater did not show any characteristic feature of any asexual reproduction of Oomycete infection on crustacean. Consequently, slide culture was performed for a more detailed examination, where the fungus isolate showed septate hyphae and vesicle-like fruiting body only upon staining via Iodine-Glycerol stain. Based on the morphological characteristic features, the present isolate was determined as a non-oomycete organism and designated as IPMB LE01 strain. According to the ITS nucleotide sequence analyses of IPMB LE01, it has matched to Zasmidium musae. This species was mainly reported in plant fungal infection and rarely observed in aquatic animals. Hence, it is considered as the first report of fungus-associated on the moribund eggs of ornate spiny lobster in Sabah.

Item Type: Experiment
Additional Information: Fungal infections were the main hindrance in crustacean seed production. This was the first study to identify the fungus species isolated from ornate spiny lobster (P. ornatus) eggs in Malaysia. Colour change in egg mass of crustacean may provide a clue to detect fungal infection. Morphological observation of IPMB LE01 showed that the fungus isolate differed to any marine Oomycetes which were widely reported in crustacean fungal infection. When based on molecular identification, IPMB LE01 was identified as Z. musae which was never reported in aquatic animals. This study suggested that Z. musae was associated with the moribund lobster eggs. Hence, further study on conidiogenesis of IPMB LE01, pathogenicity tests, and genetic characterisation using different genes are recommended
Uncontrolled Keywords: Fungus infection , Panulirus ornatus , Eggs , Zasmidium musae , First report , Sabah
Subjects: Q Science > Q Science (General) > Q1-390 Science (General)
Q Science > QK Botany > QK1-989 Botany
Divisions: INSTITUTE > Borneo Marine Research Institute
Depositing User: SITI AZIZAH BINTI IDRIS -
Date Deposited: 10 Dec 2020 16:57
Last Modified: 20 Sep 2021 08:53
URI: http://eprints.ums.edu.my/id/eprint/26452

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