Antitumor activities of spray-dried powders with different molecular masses fractionated from the crude protein-bound polysaccharide extract of Agaricus blazei Murill

Joo, Heon Hong and Joong, Joong Kim and Pogaku, Ravindra and Kwang, S. Youn (2007) Antitumor activities of spray-dried powders with different molecular masses fractionated from the crude protein-bound polysaccharide extract of Agaricus blazei Murill. Food Science and Biotechnology, 16 (4). pp. 600-604. ISSN 1226-7708

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Abstract

In this study, we first prepared 3 kinds of powders with different molecular masses from the crude protein-bound polysaccharide extract of Agraricus blazei Murill through ultrafiltration, followed by spray-drying. Then, the antitumor activities of the powders were analyzed. Size exclusion chromatography coupled with a multi-angle laser-light-scattering system showed the 3 powders had the following molecular ranges: below 10 kDa (SD-1), 10 to 150 kDa (SD-2), and above 150 kDa (SD-3), representing peak molecular weights of 8.26×103, 9.65×104, and 5.94×10 6 g/mol, respectively. All the powders stimulated macrophage RAW264.7 cells to produce nitric oxide, of which SD-2 and SD-3 were superior to the crude extract powder (CP-SD), while SD-I showed the lowest activity. Similar results were found for their cytotoxicities against human cancer cell lines (A549, MCF-7, and AGS), where the highest activity was obtained with the SD-2 treatment for 72 hr at 1,000 μg/mL. The MCF-7 cell line was less sensitive to the powders than the other cells. From this research we found that ultrafiltration, in combination with spray-drying, is applicable for preparing protein-bound polysaccharide powders with higher antitumor activities. © The Korean Society of Food Science and Technology.

Item Type: Article
Uncontrolled Keywords: Agaricus blazei Murill, Antitumor activity, Molecular weight distribution, Protein-bound polysaccharide, Ultrafiltration
Subjects: S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB320-353.5 Vegetables
R Medicine > RS Pharmacy and materia medica > RS153-441 Materia medica > RS160-167 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Divisions: SCHOOL > School of Engineering and Information Technology
Depositing User: ADMIN ADMIN
Date Deposited: 18 Apr 2011 12:42
Last Modified: 16 Oct 2017 12:01
URI: http://eprints.ums.edu.my/id/eprint/2793

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