Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides

Maria E. Sarmiento and Kai Ling Chin and Nyok Sean Lau and Ismail Aziah and Noraznawati Ismail and Mohd Nor Norazmi and Armando Acosta and Nik Soriani Yaacob (2021) Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides. Fish and Shellfish Immunology, 117. pp. 148-156. ISSN 1050-4648

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Abstract

Horseshoe crabs (HSCs) are living fossil species of marine arthropods with a long evolutionary history spanning approximately 500 million years. Their survival is helped by their innate immune system that comprises cellular and humoral immune components to protect them against invading pathogens. To help understand the genetic mechanisms involved, the present study utilized the Illumina HiSeq platform to perform transcriptomic analysis of hemocytes from the HSC, Tachypleus gigas, that were challenged with lipopolysaccharides (LPS). The highthroughput sequencing resulted in 352,077,208 and 386,749,136 raw reads corresponding to 282,490,910 and 305,709,830 high-quality mappable reads for the control and LPS-treated hemocyte samples, respectively. Based on the log-fold change of > 0.3 or < -0.3, 1338 genes were significantly upregulated and 215 genes were significantly downregulated following LPS stimulation. The differentially expressed genes (DEGs) were further identified to be associated with multiple pathways such as those related to immune defense, stress response, cytoskeleton function and signal transduction. This study provides insights into the underlying molecular and regulatory mechanisms in hemocytes exposed to LPS, which has relevance for the study of the immune response of HSCs to infection.

Item Type: Article
Uncontrolled Keywords: Tachypleus gigas , Horseshoe crab , Hemocytes , Lipopolysaccharide challenge , Infection , Immune response , Innate immunity , Transcriptomics , DEGs , High-throughput sequencing , RNA-Seq
Subjects: Q Science > QL Zoology > QL1-991 Zoology
Q Science > QR Microbiology > QR1-502 Microbiology
Divisions: FACULTY > Faculty of Medicine and Health Sciences
Depositing User: DG MASNIAH AHMAD -
Date Deposited: 29 Oct 2021 10:37
Last Modified: 29 Oct 2021 10:37
URI: http://eprints.ums.edu.my/id/eprint/30884

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