Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy

Matsui, Masafumi and Amir Hamidy and Daicus M. Belabut and Norhayati Ahmad and Panha, Somsak and Ahmad Sudin and Khonsue, Wichase and Oh, Hong Shik and Yong, Hoi Sen and Jiang, Jian ping and Nishikawa, Kanto (2011) Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylogenetics and Evolution, 61 (1). pp. 167-176. ISSN 1055-7903


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We estimated the genealogical relationships and assessed systematic relationships among 45 out of 89 named species and four unnamed taxa from 11 of 14 genera of the Oriental microhylids from 1767. bp sequences of the mitochondrial DNA genes 12S rRNA and 16S rRNA using maximum parsimony, maximum likelihood, and Bayesian inference methods. Monophyly was rejected for the subfamily Microhylinae, and our data reveal four well-supported clades whose relationships to each other are unresolved: (A) Microhyla, Calluella, and Glyphoglossus, (B) Chaperina, (C) Kaloula, Phrynella, and Metaphrynella, and (D) Micryletta. They were genetically as divergent from each other as from another Oriental subfamily Kalophryninae, and could be recognized as distinct subfamilies. Within Clade A, our data reveal three well-supported subclades whose relationships to each other are unresolved: (AI) Microhyla-I, (AII) Calluella and Glyphoglossus, and (AIII) Microhyla-II. Of the two enigmatic Malaysian genera, whose subfamilial placement has been undetermined, Phrynella was found to be the sister species of Metaphrynella in Clade C, whereas Gastrophrynoides was grouped in the Papua-Australian subfamily Asterophryinae. Currently recognized subgenera and species groups within Microhyla based on morphology were not supported phylogenetically, and require thorough reassessments.

Item Type: Article
Uncontrolled Keywords: Asia, Gastrophrynoides, MtDNA, Papua-Australian element, Phrynella, Subgeneric classification, Mitochondrial DNA, Ribosome RNA, RNA 16S, RNA, Ribosomal, 12S, Animal, Article, Bayes theorem, Classification, DNA sequence, Frogs and toads, Genetics, Mitochondrion, Molecular evolution, Nucleotide sequence, Pedigree, Phylogeny
Subjects: Q Science > QL Zoology > QL1-991 Zoology > QL605-739.8 Chordates. Vertebrates > QL640-669.3 Reptiles and amphibians
Divisions: INSTITUTE > Institute for Tropical Biology and Conservation
Depositing User: ADMIN ADMIN
Date Deposited: 18 Sep 2012 15:07
Last Modified: 17 Oct 2017 15:26

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