Anthocyanin analysis of Vanda mericlone using HPLC

Leong, Wai Ting (2005) Anthocyanin analysis of Vanda mericlone using HPLC. Universiti Malaysia Sabah. (Unpublished)


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he objective of this research was to discover the quantity and quality of anthocyanin and analyzing the correlation between the quantity of types of anthocyanins and the ability of producing floral fragrant by Vanda sp. The seedlings were grown under same condition; 24 hours exposed to light with 25±2ºC. In the extraction process, 0.1 % (v/v) hydrochloric acid in methanol acid solution used to extract anthocyanins. After the extracting process, high performance liquid chromatography was used to identify and measure the amount of six main types of anthocyanins; delphinidin, cyanidin, petunidin, pelargonidin, peonidin and malvidin. The peaks of the chromatograms for each sample were identified by comparing the absorption spectra and retention to that of authentic standards. The area under the curve of the wanted peaks used to fmd out the amount of the particular anthocyanins in the Vanda sp. seedlings. The results shown that delphinidin was not found in the Vanda sp. seedlings. The sample No.36 of Vanda dearei had the highest content of malvidin and peonidin, while sample No.35 of Vanda dearei had the highest amounts of pelargonidin and No.22 was found to contain the highest amount of petunidin. Besides, among 50 seedlings of Vanda helvola, the samples No. 25 produced the highest amount of petunidin and pelargonidin. While sample No.7 had the highest content of peonidin and No.28 has the highest content of malvidin. In conclusion, it was proven that the types of anthocyanins produced by Vanda sp. was suitable to be analyzed by using HPLC and the particular types of anthocyanins gave the colours to the flowers of the adult plant. Meanwhile, the seedlings composed of high level of malvidin may have the potential in producing fragrance during blooming in adult. The result of this research will be very beneficiary to expand on further study on variations plants in order to observe the relationship between floral fragrance and the amount of malvidin produced.

Item Type: Academic Exercise
Uncontrolled Keywords: quantity and quality, anthocyanin, floral fragrance, Vanda sp., extraction process, malvidin
Subjects: Q Science > QK Botany
Q Science > QD Chemistry
Divisions: SCHOOL > School of Science and Technology
Date Deposited: 03 Sep 2013 11:23
Last Modified: 23 Oct 2017 16:10

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