Jualang Azlan Gansau and Goh, Keng Sean (2009) Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia. Borneo Science, 21. pp. 37-46. ISSN 1394-4339
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Abstract
Studies on eukaryotic signal transduction pathways have led to a variety of potential molecular targets for cancer therapy including Mitogen-activated Protein Kinase Kinase 1 (MKK1), Glycogen Synthase Kinase 3ß (GSK-3ß) and Type 1 Protein Phosphatase (PP1). The protein kinases and phosphatases in the signal transduction pathways play a vital role in mitogen activated protein (MAP) kinase signal transduction, tumorigenesis, apoptosis, and cancer metastasis. In this study, enetically engineered yeast strains were used as a model in the search for potential inhibitors against these signalling elements. Eleven species of medicinal plants were selected from various locations in Sabah, Malaysia, extracted and tested against MKK, GSK-3ß and PP1. Preliminary results showed the presence of potential MKK1 inhibitors in the crude extracts of Alphitonia excelsa, Cordyline terminalis and Tinospora crispa. However, no inhibitor was found against GSK-3ß and PP1. The crude extracts of Cordyline terminalis was further fractionated using a bioassay guided fractionation and four partially pure active fractions were isolated.
| Item Type: | Article |
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| Keyword: | Inhibitor, Kinase, Phosphatase, Signal transduction, Medicinal plants |
| Subjects: | Q Science > QR Microbiology |
| Department: | FACULTY > Faculty of Science and Natural Resources |
| Depositing User: | NORAINI LABUK - |
| Date Deposited: | 09 Mar 2018 15:16 |
| Last Modified: | 09 Mar 2018 15:16 |
| URI: | https://eprints.ums.edu.my/id/eprint/19240 |
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