Jualang Azlan Gansau and Marziah Mahmood (2013) Growth Characteristics and Production of Physalins from Physalis minima Hairy Roots in Shake Flasks. Kasetsart Journal: Natural Science, 47 (5). pp. 748-759. ISSN 2452-316X
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Abstract
This report described the growth characteristics and production of physalins from Physalis minima hairy roots in shake fl ask culture. The presence of lateral branches in the inoculum had a negligible effect on the fi nal root biomass dry weight (DW), specifi c growth rate (μ), doubling time (td) and production of physalins. However, excising the primary and lateral root tips reduced μ and the total root length but not the fi nal biomass. Mature root tissues were observed to accumulate more physalin B and F (1.55 and 3.74 mg.g-1 DW, respectively) compared to the root tips (0.65 and 1.47 mg.g-1 DW, respectively). Increasing the number of root tips from 2 to 12 and the medium volume signifi cantly reduced μ and extended td. Decreasing the medium volume with a small number of inocula reduced td, improved the biomass and production of physalins and μ. Using a 100 mL fl ask, four root tips cultured in 25 mL medium provided the optimum conditions for biomass (0.24 g DW) and production of physalins (1.68–3.5 mg.g-1 DW).
| Item Type: | Article |
|---|---|
| Keyword: | Physalis minima, hairy roots, physalin, inoculum morphology, inoculum size, medium volume |
| Subjects: | Q Science > QK Botany |
| Department: | CENTRE > Centre for the Promotion of Knowledge and Language Learning |
| Depositing User: | MUNIRA BINTI MARASAN - |
| Date Deposited: | 21 Jun 2018 13:52 |
| Last Modified: | 21 Jun 2018 13:52 |
| URI: | https://eprints.ums.edu.my/id/eprint/20297 |
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