Mohamed Qasim Al-Fahdawi and Faris A.J. Al-Doghachi and Qasim Khlaif Abdullah and Ruaa Tareq Hammad and Abdullah Rasedee and Wisam Nabeel Ibrahim and Hussah Abdullah Alshwyeh and Areej A Alosaimi and Sahar Khamees Aldosary and Eltayeb E. M. Eid and Rozita Rosli and Y.H. Taufiq-Yap and Nagi A. Al-Haj and Mothanna Sadiq Al-Qubaisi (2021) Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells. Biomedicine & Pharmacotherapy, 138. pp. 1-11.
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Abstract
The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinumdoped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive Xray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/ MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anticancer compound.
| Item Type: | Article |
|---|---|
| Keyword: | Pt/MgO nanoparticles, Cytotoxicity, Oxidative stress, Lipid peroxidation, Apoptosis, Lung cancer, Colonic cancer |
| Subjects: | R Medicine > R Medicine (General) |
| Department: | FACULTY > Faculty of Science and Natural Resources |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 14 Apr 2021 09:12 |
| Last Modified: | 15 Apr 2021 13:54 |
| URI: | https://eprints.ums.edu.my/id/eprint/26750 |
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