Detaction of genetic in selected Sabah Park’s germplasm collection of slipper orchids (Paphiopedilum rothschildianum) using RAPD markers

Wong, Kian Yong (2004) Detaction of genetic in selected Sabah Park’s germplasm collection of slipper orchids (Paphiopedilum rothschildianum) using RAPD markers. Universiti Malaysia Sabah. (Unpublished)

[img]
Preview
Text
ae0000000446.pdf

Download (2MB) | Preview

Abstract

Random Amplified Polymorphic DNA CRAPO) markers were used to detect genetic variation in selected Taman Sabah's gennplasm collection of Paphiopedianum rothschildianum, an endangered slipper orchid in Sabah. Four populations of the species, which obtained from the locations including Penataran, Telupid, Kota Belud and Ampuan, were selected in the studied. A total of 46 amplified bands were scored from five RAPD markers and a mean of 9.2 per primer and the percentage of polymorphic bands was 93.48%. All populations of the species were found to share one or more RAPD markers with five primers. The cluster analysis of distance values was conducted to construct a dendrogram. The study revealed a moderate level of genetic polymorphism and variability among populations in P. rothschildianum and no direct relatonship between geographical distance and genetic variation between the four populations of P. rothschildianum.

Item Type: Academic Exercise
Keyword: Slipper orchid, Paphiopedianum rothschildianum, Random Amplified Polymorphic DNA, Sabah Park, Penataran, Telupid, Kota Belud, Ampuan
Subjects: Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms
Department: SCHOOL > School of Science and Technology
Depositing User: ADMIN ADMIN
Date Deposited: 17 Jan 2012 17:21
Last Modified: 23 Oct 2017 08:26
URI: https://eprints.ums.edu.my/id/eprint/3319

Actions (login required)

View Item View Item