Teh, Ning Wei (2017) Effect of organic soil amendments on growth, phytochemical content and antimicrobial activity of Sabah Snake Grass (Clinacanthus Nutans). Universiti Malaysia Sabah. (Unpublished)
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Abstract
Clinacanthus nutans has been used to treat various diseases in Asian countries. The leaves of C nutans contain high level of antioxidant activity which is important in the protective against oxidative damage of human body. The study was conducted under the rainshelter and laboratory of Faculty of Sustainable Agriculture, Universiti Malaysia Sabah to determine the effect of organic soil amendments on growth, phytochemical content and antimicrobial activity of Sabah Snake Grass ( Clinacanthus nutans). This research was conducted using Completely Randomized Design (CRD). Six treatments which are rice husk, rice bran, cocopeat, empty fruit bunch (EFB), spent mushroom substrate (SMS) and a control with five replicates for each treatment make a total of 30 samples. The results were statistical analysed using SPSS® version 21 software package. One-way Analysis of Variance (ANOVA) followed by Tukey test was carried out to determine the significance between means. The values were considered to be significantly different when P<0.05. The growth development of C nutans showed significant differences (P<0.05) in plant height. The plant height of C nutans was found to be highest that grow in the EFB amendment (45.06 ± 11.01 cm). The highest total phenolic content of C nutans was recorded in the rice husk treatment (10.55 ± 1.68 mg GAE/g), while the highest total flavonoid content of C nutans was recorded in the SMS treatment (11.75 ± 2.84 mg QE/g). The planting of C nutans in SMS amendment recorded the highest DPPH radical scavenging activity with IC50 of 6914.32 µg/ml. It was observed that only C nutans that treated with rice bran amendment exhibit antimicrobial activity against Escherichia coli (6.17 ± 0.29 mm). Next, C nutans that planted in control treatment showed the highest antimicrobial activity against Staphylococcus aureus (9.67 ± 1.53 mm) followed by rice husk (8.33 ± 0.58 mm), and cocopeat amendment (8.00 ± 1.00 mm). SMS and rice husk amendments are potential for the improvement of total phenolic and total flavonoid content to enhance human health. Thus, different application rate of SMS and rice husk amendments in the growing media can be conducted to improve the phytochemical content and maximum yield production of C nutans.
| Item Type: | Academic Exercise |
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| Keyword: | Organic amendments; spent mushroom substrate; rice husk; antioxidant; phenolic; flavonoid; antimicrobial; Clinacanthus nutans |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | NORAINI LABUK - |
| Date Deposited: | 09 Jan 2020 07:32 |
| Last Modified: | 09 Jan 2020 07:32 |
| URI: | https://eprints.ums.edu.my/id/eprint/24592 |
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