Mailin Misson and Bo Jin and Sheng Dai and Hu Zhang (2020) Interfacial biocatalytic performance of nanofiber-supported β-galactosidase for production of galacto-oligosaccharides. Catalysts, 10 (1). p. 81. ISSN 2073-4344
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Abstract
Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at a residence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.
Item Type: | Article |
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Keyword: | β-galactosidase , polymer nanofibers , nanobiocatalyst , interfacial characterization , interfacial reaction , conformational change |
Subjects: | T Technology > TP Chemical technology |
Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
Depositing User: | NORAINI LABUK - |
Date Deposited: | 12 Jun 2020 07:53 |
Last Modified: | 08 Apr 2021 22:50 |
URI: | https://eprints.ums.edu.my/id/eprint/25511 |
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