Tien‐Tien Vicky Lau and Suat‐Moi Puah and Ching, Kee Karen Hon and Ching, Fui Fui and Jin Ai Mary Anne Tan and Savithri Devi Ampalam Puthucheary and Lee, Ping Chin and Kek Heng Chua (2020) Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia. Aquaculture Research, 51 (10).
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Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia.pdf Download (44kB) | Preview |
Abstract
Aeromonads are ubiquitous in aquatic environments and have been implicated in fish and human infections. In this study, we isolated, studied antimicrobial susceptibility patterns and screened the existence of 15 virulence genes in aeromonads from two famously consumed fish species—seven marine Tiger Grouper (Epinephelus fuscoguttatus) and eight freshwater Marble Goby (Oxyeleotris marmoratus) from the aquaculture hatchery in Sabah, Malaysia. A total of 30 aeromonads (17 A. caviae, 9 A. rivuli, 4 A. dhakensis) were identified using PCR targeting GCAT gene, rpoD‐restriction fragment length polymorphism and multi‐locus phylogenetic analysis. All 30 strains were resistant to amoxicillin and cephalothin and five strains were multidrug‐resistant. Nine virulence genes (lip, ela, eno, fla, aerA, hylA, dam, alt and ser) present in A. dhakensis, suggesting the virulence potential of this species as a fish pathogen. This study offers as a baseline for future studies in monitoring and managing these two fish in aquaculture industry.
| Item Type: | Article |
|---|---|
| Keyword: | Tiger Grouper,Marble Goby,Sabah |
| Subjects: | Q Science > Q Science (General) S Agriculture > SH Aquaculture. Fisheries. Angling |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 24 Sep 2020 13:40 |
| Last Modified: | 24 Sep 2020 13:40 |
| URI: | https://eprints.ums.edu.my/id/eprint/26018 |
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