Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii

Mohd Khalizan Sabullah and Mohd Ezuan Khayat (2015) Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii. Journal of Biochemistry, Microbiology and Biotechnology, 3 (2). pp. 26-29. ISSN 2289-5779

Text Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii FULL TEXT.pdf Restricted to Registered users only Download (234kB) | Request a copy

Abstract

In this study, the substrate specificity and the inhibition kinetics of various types of insecticides to the acetylcholinesterase (AChE) from a local fish; Puntius schwanenfeldii were investigated. The substrate specificity determination was done using three thiocholine substrates, which were ATC, PTC and BTC. The results showed that he partially purified cholinesterase from Puntius schwanenfeldii that preferred ATC is a true AChE. The Km and Vmax values of AChE for these substrates were 16.61 mmol and 286.5 U/mg for ATC, 19.92 mmol and 245.3 U/mg for PTC, and 48.64 mmol and 219.6 U/mg for BTC, respectively. The IC50 values for the carbamates bendiocarb, carbaryl, propoxur, carbofuran and methomyl were 0.838, 7.045, 29.441, 1.411 and 8.335 mg/L, respectively, which were comparable to the IC50 values for carbamates from several AChE from fish.

Item Type:	Article
Keyword:	Acetylcholinesterase , Fish , Puntius Schwanenfeldii , In Vitro Assay , Carbamates
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301-705.5 Biology (General) S Agriculture > SH Aquaculture. Fisheries. Angling
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	29 Jun 2021 07:37
Last Modified:	29 Jun 2021 07:37
URI:	https://eprints.ums.edu.my/id/eprint/27477

Actions (login required)

View Item