Characterisation of cholinesterase activity from Asian swamp eel; monopterus albus, and testing its capabilities as a biosensor on metal ion contamination

Siti Aishah Muhammad Khalidi and Nureen Nordina and Nur Hazrina Nurizan and Rahmath Abdulla and Mohd Yunus Shukor and Siti Aqlima Ahmad and Mohd Rosni Sulaiman and Mohd Khalizan Sabullah (2020) Characterisation of cholinesterase activity from Asian swamp eel; monopterus albus, and testing its capabilities as a biosensor on metal ion contamination. Jurnal Teknologi, 82. pp. 157-164. ISSN 2180-3722

	Text Characterisation of cholinesterase activity from Asian swamp eel.pdf Download (43kB)
	Text Characterisation of cholinesterase activity from Asian swamp eel1.pdf Restricted to Registered users only Download (467kB) \| Request a copy

URL: https://journals.utm.my/jurnalteknologi/article/vi...

Abstract

The presence of high concentrations of heavy metals in the river impedes daily activities and damages the surrounding aquatic ecosystem. Continuous monitoring should be implemented to prevent continuous discharge resulting in increased levels of pollution over time. This study aims to determine the sensitivity of Monopterus albusmuscle ChE to metal ions. Priorly, ChE was purified from M. albusmuscle tissueusing the ion exchange matrix, DEAE, with the yield percentage of 42.16% with the purification fold of 2.17. PTC was selected as a specific synthetic substrate with the highest concentration and lowest biomolecular constant at 145838 ± 7533 μM.min.-1mg.-1 and 0.26 mM, while optimal assay parameters were obtained at pH 7.5 at 20 ° C. Inhibition studies of metal ions involving Cr, Cd, Ag, As, Hg, Pb, Ni and Cu at the concentration of 10 mg/L. The activity of M. albusChE was 56.61% inhibited by Hg2+and the highest recorded compared to the other metals ion arranged in declining order; Cr <Cd = Ni <Cu <Pb <As <Ag. Overall, purified ChE from M. albusmuscle has proven its ability to be applied as a biosensor that can be used for environmental monitoring programs.

Item Type:	Article
Keyword:	Pollution , Metal ions , Biosensor , Cholinesterase , Optimal assay
Subjects:	Q Science > QL Zoology > QL1-991 Zoology > QL605-739.8 Chordates. Vertebrates > QL614-639.8 Fishes Q Science > QR Microbiology > QR1-502 Microbiology > QR100-130 Microbial ecology
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	06 Dec 2021 08:00
Last Modified:	06 Dec 2021 08:00
URI:	https://eprints.ums.edu.my/id/eprint/31358

Actions (login required)

View Item