Phytochemical constituents, antioxidant and antiproliferative properties of a liverwort, lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia

Mohd Fadzelly Abu Bakar and Fifilyana Abdul Karim and Monica Suleiman and Azizul Isha and Asmah Rahmat (2015) Phytochemical constituents, antioxidant and antiproliferative properties of a liverwort, lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia. Evidence-Based Complementary and Alternative Medicine, 2015. pp. 1-9. ISSN 1741-4288

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Abstract

The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents.

Item Type:	Article
Keyword:	Phytochemical , Antioxidant , Antiproliferative , Liverwort , Lepidozia borneensis , Mount Kinabalu , Sabah
Subjects:	Q Science > QK Botany > QK1-989 Botany > QK504-(638) Cryptogams
Department:	INSTITUTE > Institute for Tropical Biology and Conservation
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	06 Apr 2022 12:16
Last Modified:	06 Apr 2022 12:16
URI:	https://eprints.ums.edu.my/id/eprint/32224

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