In vitro release and cytotoxicity study of encapsulated sulfasalazine within LTSP micellar/ liposomal and TSP micellar/niosomal nano-formulations

Mohammad Javed Ansari and Mohammed F. Aldawsari and Ameeduzzafar Zafar and Alireza Soltani and Mohd Yasir and Mohammed Asadullah Jahangir and Mohamad Taleuzzaman and Vahid Erfani-Moghadam and Leila Daneshmandi and Nosrat O Mahmoodi and Asieh Yahyazadeh and Md Lutfor Rahman and Mohd Sani Sarjadi (2022) In vitro release and cytotoxicity study of encapsulated sulfasalazine within LTSP micellar/ liposomal and TSP micellar/niosomal nano-formulations. Alexandria Engineering Journal, 61. pp. 9749-9756. ISSN 1110-0168

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URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

The micelles/liposome formulation for the first time has been constructed via thin-film hydration method containing soy lecithin (L), tween 80 (T), squalene (S), and polyvinyl alcohol (P) (LTSP nanoparticles). Similar ingredients except for lecithin were used for preparing micellar/niosomal vesicular SSZ nano formulation (TSP nanoparticles). The percent drug loading and encapsulation efficiency of SSZ was 7.39% and 98.5 ± 0.3 % for the 7.5:100 (w/w) ratio of SSZ: total weight of LTSP, while the percent drug loading and encapsulation efficiency of SSZ was 4.7% and 62.85 ± 0.3 % in the TSP nano formulation. Dynamic light scattering (DLS) and trans- mission electron microscopy (TEM) results showed that both formulations formed spherical micelles and vesicles with globule sizes of 25 ± 1.2 nm and 100 ± 20.5 nm respectively. The cell toxicity evaluations showed that both LTSP and TSP nano formulations without drug were nontoxic (at the range of this experiment) for Human Dermal Fibroblasts (HDF) as a normal cell line, but SSZ loaded nano formulation exhibited increased cell toxicity with half-maximal inhibitory concentration (IC50) of 940 mM for SSZ alone to near 240 mM for SSZ loaded nano formulation (approximately four times). In vitro release experiments exhibited sustained release of SSZ from both nano formulations. The LTSP micellar/liposomal and TSP micellar/niosomal nano formulation for SSZ delivery can be considered as appropriate approaches for improving its bioavailability and probably they are good candidates for future clinical investigations.

Item Type:	Article
Keyword:	Micelles , Niosomes , Liposomes , Sulfasalazine , Drug Delivery , Bioavailability
Subjects:	Q Science > QD Chemistry > QD1-999 Chemistry
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	20 May 2022 08:40
Last Modified:	20 May 2022 08:40
URI:	https://eprints.ums.edu.my/id/eprint/32639

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