Mariam Abd Latip, Datin and Zakri A. H. and Mahani M. C. and Normah M. N. (1996) Interspecific hybridization of cultivated rice, Oryza sativa L. with the wild rice, O. minuta Presl. Theoretical and Applied Genetics, 93 (5-6). pp. 664-671. ISSN 00405752
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Abstract
Crosses were made between four varieties ('Mahsuri', 'Setanjung', 'MR84' and 'MR103') of Oryza sativa L. (2n = 24, AA) and one accession of O. minuta (2n = 48, BBCC). The seed set obtained ranged between 9.5% and 25.1% depending on the rice variety used. By rescuing 14-day-old embryos and culturing them on 25%-strength MS medium we obtained a total of 414 F1 hybrids. The F1s were vigorous, tillered profusely, were perennial and male-sterile. The hybrids were triploid (ABC) with 36 chromosomes and showed irregular meiosis. The average frequency and range of chromosome associations at metaphase I or early anaphase I pollen mother cells of F1 plants were 29.31(16-36) Is + 3.32(0-10) IIs + 0.016(0-1) IIIs + 0.002(0-1) IIIs. Upon backcrossing the original triploid hybrids and colchicine-treated hybrids to their respective recurrent parents, and further embryo rescue, 17 backcross-1 (BC�) plants were obtained. Of all the crosses using MR84, no BC� plant was obtained even after pollinating 13894 spikelets of the triploid hybrid. The BC�s were similar in appearence to the F1S and were male-sterile, their chromosome number ranged from 44 to 48. By backcrossing these BC�s and nurturing them through embryo rescue, we obtained 32 BC₂ plants. Of these, however, only 18 plants grew vigorously. One of these plants has 24 chromosomes and the other 17 have chromosome numbers ranging between 30 and 37. The 24-chromosome plant was morphologically similar to the O. sativa parent and was partially fertile with a pollen and spikelet fertility of 58.8% and 12.5% respectively. All of the F1 and BC� plants were found to be resistant to five Malaysian isolates (XO66, XO99, XO100, XO257 and XO319) of Xanthomonas campestris pv oryzae. Amongst the BC₂s, the reaction varied from resistant to moderately susceptible. The 24-chromosome BC₂ plant was resistant to the four isolates and moderately resistant to isolate XO100 to which the O. sativa parent was susceptible.
| Item Type: | Article |
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| Keyword: | Embryo rescue, Interspecific hybridization, O. minuta, Oryza sativa, Resistance to bacterial blight |
| Subjects: | S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants |
| Department: | SCHOOL > School of Science and Technology |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 30 Jan 2012 11:01 |
| Last Modified: | 16 Oct 2017 14:23 |
| URI: | https://eprints.ums.edu.my/id/eprint/3383 |
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