Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor

Azlinah Matawali and Lee, Ping Chin and Jualang Azlan Gansau (2019) Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor. The Journal of Phytopharmacology, 8. pp. 1-4. ISSN 2320-480X

	Text Abstract.pdf Download (43kB)
	Text Full text.pdf Restricted to Registered users only Download (270kB)

Abstract

Chromolena Odorata has been traditionally used as wound healer in local community. The present study investigated the anti-kinase and anti-phosphatases activities on methanolic C. odorata extract. Mutant yeast strains used are MKK1P386, MKK1P386_MSG5, PAY704-1 and PAY700-4. Bioassay guided fractionation of C. odorata revealed positive activities of hexane, ethyl acetate and chloroform partitions. Column chromatography of all partitionates later confirmed fraction F2 from chloroform extract had most favorable activity with inhibitory zone ranged between 7±0.0 mm until 15±0.0 mm. Kinetic analysis including maximum enzyme velocity (Vm) and Michealis-Menten constant (Km) were evaluated and compared for both normal and inhibited reactions. Enzyme activity with DiFMUP as substrate showed fraction F2 act as PP1 enzyme inhibitor with the Km value 0.60 mM and Vm value 200 mM/min as compared to the normal enzymatic reaction. Results provided unveil the potential of C. odorata as an effective therapeutic agent.

Item Type:	Article
Keyword:	Chromolena Odorata , Pokok kapal terbang , Sabah , Protein phosphatase type-1
Subjects:	Q Science > QK Botany > QK1-989 Botany
Department:	CENTRE > Preparation Centre for Science and Technology
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	05 Dec 2022 08:07
Last Modified:	05 Dec 2022 08:07
URI:	https://eprints.ums.edu.my/id/eprint/34994

Actions (login required)

View Item