Bacteria-sponge interaction : Existence of possible chemical correlation

Koh, Johleen Tse Boon (2010) Bacteria-sponge interaction : Existence of possible chemical correlation. Masters thesis, Universiti Malaysia Saba.

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Abstract

The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon sp. and Xestospongia sp. and the most active sponge was then investigated for its relationship with its inherently available culturable bacteria in an effort to confirm the identity of the bioactive compound producer. All fractions except for fraction 1 and fraction 6 from the hexane crude extract from Amphimedon sp. showed antibacterial activity against 5 strains of marine environmental bacteria while all fractions except for fraction 1 from the 90% methanol crude extract showed antibacterial activity against 5 strains of marine environmental bacteria. Amphimedon sp. produced potent sterol as its bioactive compound, while Xestosponge sp produced aaptamine. Their identities were identified based _on extensive H 1-NMR and MS data. Spongebacteria relationship was carried out by isolating culturable bacteria from sponge tissues, and a total of 40 strains of bacteria were isolated from Amphimedon sp. but only one strain (AA1) was found to have bioactive activity. 16S rRNA regions of AAl were amplified using forward primer 27F (GAGTTTGATCCTGGCTCAG) while the reverse primer was JR1R (GACTACCAGGGTABCTAATC) and was compared with the top three matches provided by NCBI GenBank. Strain AA1 was found to be pro Bacillus sp. with 99% confidence and with e-value of zero. A mixture of fatty acids were isolated as active metabolite from AAl and upon gas chromatography (GC) analysis and antibacterial assay with corresponding commercial fatty acids. It was confirmed that hexadecenoate was the active compound in AAl. In conclusion, culturable bacteria were not responsible for the production of active compounds in Amphimedon sp.

Item Type:	Thesis (Masters)
Keyword:	Bacteria, Marine sponges, Ocean, Bioactive compound
Subjects:	Q Science > QL Zoology > QL1-991 Zoology > QL360-599.82 Invertebrates
Department:	INSTITUTE > Borneo Marine Research Institute
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	26 Jan 2024 15:20
Last Modified:	26 Jan 2024 15:20
URI:	https://eprints.ums.edu.my/id/eprint/38031

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