The Potential Chemo Preventive Effect of Andrographis paniculata on 1,2- Dimethylhydrazine and High-Fat-Diet-Induced Colorectal Cancer in Sprague Dawley Rats

Tharani Subarmaniam and Rusydatul Nabila Mahmad Rusli and Kokila Vani Perumal and Yoke Keong Yong and Siti Hadizah and Fezah Othman and Khaled Salem and Nurul Husna Shafie and Rosnani Hasham and Khoo Boon Yin and Khairul Kamilah Abdul Kadir and Hasnah Bahari and Zainul Amiruddin Zakaria (2023) The Potential Chemo Preventive Effect of Andrographis paniculata on 1,2- Dimethylhydrazine and High-Fat-Diet-Induced Colorectal Cancer in Sprague Dawley Rats. International Journal of Molecular Sciences, 24. pp. 1-17. ISSN 1422-0067

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URL: https://www.mdpi.com/1422-0067/24/6/5224#metrics

Abstract

Colorectal cancer (CRC) is responsible for a notable rise in the overall mortality rate. Obesity is found to be one of the main factors behind CRC development. Andrographis paniculata is a herbaceous plant famous for its medicinal properties, particularly in Southeast Asia for its anti-cancer properties. This study examines the chemopreventive impact of A. paniculata ethanolic extract (APEE) against a high-fat diet and 1,2-dimethylhydrazine-induced colon cancer in Sprague Dawley rats. Sprague Dawley rats were administered 1,2-dimethylhydrazine (40 mg/kg, i.p. once a week for 10 weeks) and a high-fat diet (HFD) for 20 weeks to induce colorectal cancer. APEE was administered at 125 mg/kg, 250 mg/kg, and 500 mg/kg for 20 weeks. At the end of the experiment, blood serum and organs were collected. DMH/HFD-induced rats had abnormal crypts and more aberrant crypt foci (ACF). APEE at a dose of 500 mg/kg improved the dysplastic state of the colon tissue and caused a 32% reduction in the total ACF. HFD increased adipocyte cell size, while 500 mg/kg APEE reduced it. HFD and DMH/HFD rats had elevated serum insulin and leptin levels. Moreover, UHPLC-QTOF-MS analysis revealed that APEE was rich in anti-cancer phytochemicals. This finding suggests that APEE has anti-cancer potential against HFD/DMH-induced CRC and anti-adipogenic and anti-obesity properties.

Item Type:	Article
Keyword:	Andrographis paniculata, Colorectal cancer, CRC, High-fat diet, HFD, DMH; obesity, Anti-cancer
Subjects:	Q Science > Q Science (General) > Q1-390 Science (General) > Q1-295 General R Medicine > RS Pharmacy and materia medica > RS153-441 Materia medica
Department:	FACULTY > Faculty of Medicine and Health Sciences
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	23 Apr 2024 15:07
Last Modified:	23 Apr 2024 15:07
URI:	https://eprints.ums.edu.my/id/eprint/38574

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