Elaine Remi Anak Douglas Telajan (2009) Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Palm kernel pulp (PKC) is one of the important waste products of palm oil. PKC has been used as feed for ruminant animals and poultry and ducks. The use of PKC in general poultry and duck feed is limited due to the anti-nutritional properties such as galactomannan and xylan found in PKC. These anti-nutritional properties increase the viscosity of the diet due to high water absorption and this will limit the absorption of nutrients by farm animals. . One way to overcome this problem is to treat PKC with enzymes or microorganisms that produce enzymes that can degrade rigid components to nutritional value. Therefore, this project was implemented to isolate mesophilic and thermophilic bacteria and screen them in their ability to produce galactomannanase, cel/ulase, xylanase and lipase enzymes. These enzymes can improve the nutritional quality of PKC as a single cell protein {SCP}. Isolation is done using the 'dilution plate' method on Nutrient agar for bacteria, Starch Casein Nitrate agar {SCA) for mesophilic actinomycetes, Czapexdox Yeast Extract Casamino Acid agar {CYC) for thermophilic actinomycete, yeast extract peptone glucose agar (YEPD) for yeast and potato dextrose agar (PDA) for fungi. Commercial substrate; Azo-carob-galactomannan, Azo-xylan (oat) and AzoCM- cel/ulose were used to screen galactomannanase, xylanase and cellu/ase while sobitan monolaurate {Tween 20) was used as a substrate for lipase activity. A total of 1146 microorganisms were isolated from several sources of "empty fruit bunch" (EFB) compost, effective microbial solutions and raw EFB. Of these, 627 bacteria, 219 actinomycete, 101 yeasts and 199 fungi were isolated and screened. Six isolates with the ability to produce mannanase, ce/lulase, xylanase enzymes were quantitatively calculated mannanase, cellu/ase and xylanase activities. Found three separate Bacillus sp. different ones give mannanase activity {Isolates 7DY7, 7DU3 and 4DB3 respectively with a maximum activity of 1.30 U/μg protein, 0.95 U/μg protein and 0.92 U/μg protein), cellulase {Isolates 7DY7, 7DU3 and 4DB13 respectively with maximum activity of 0.08 U/μg protein, 0.35 U/μg protein and 0.11 U/μg protein) and xylanase (Isolate 7DY7, 7DU3 and 4D88 respectively with maximum activity of 0.15 U/μg protein, 0.08 U/μg protein and 0.21 U/μg protein) which is relatively high when compared to 13 other bacterial isolates. The six bacterial isolates were sought to be identified using their respective 165 rDNA sequences. Eleven Bacillus sp. and a single isolate of Micromonospora sp., Streptomyces sp. and Thermoactinomyces sp. each identified by comparing the partial 16S rDNA sequence of the isolates with the 16S rDNA sequence found in GenBank using the Basic Alignment Search Tool (BLAST). Microorganisms isolated can be used to treat PKC to increase the nutritional value of food in PKC
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Palm kernel pulp, PKC, Oil palm by-products, Animal feed |
| Subjects: | S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 10 May 2024 10:52 |
| Last Modified: | 10 May 2024 10:52 |
| URI: | https://eprints.ums.edu.my/id/eprint/38637 |
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