DNA barcoding using chloroplast matK and rbcL regions for the identification of bamboo species in Sabah

Thau, Wilson Lym Yong and Anis Adilah Mustafa and Mohammad Rahmat Derise and Kenneth Francis Rodrigues (2024) DNA barcoding using chloroplast matK and rbcL regions for the identification of bamboo species in Sabah. Advances in Bamboo Science, 7. pp. 1-10. ISSN 2773-1391

	Text ABSTRACT.pdf Download (39kB)
	Text FULL TEXT.pdf Restricted to Registered users only Download (8MB) \| Request a copy

URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

Bamboo species play pivotal roles in maintaining ecological diversity and driving socio-economic development in Sabah, Malaysia. However, due to overlapping traits among distinct bamboo species, identifying them based solely on morphological characteristics poses challenges. DNA barcoding emerges as a highly effective method, demonstrating potential accuracy in identifying diverse plant species across various taxa and ecosystems. In this study, we used the chloroplast matK and rbcL regions as barcode markers to identify 15 bamboo plants in Sabah. Both matK and rbcL exhibited a notable 100% accuracy at the genus level, indicating their reliability in capturing broader taxonomic categories during BLAST searches and genetic distance analysis. However, at the species level, the success rate was limited to 60%, revealing challenges in differentiating closely related bamboo species using the existing DNA barcodes. Our findings underscore the need for an advanced DNA barcode reference library with extensive species coverage for accurate species identification. This study not only unveiled crucial insights into bamboo diversity and taxonomy in Sabah but also contributes to ongoing bamboo resource conservation and supports sustainable resource management in the region.

Item Type:	Article
Keyword:	Bamboo species, DNA barcoding, matK, rbcL, Species identification
Subjects:	Q Science > QK Botany > QK1-989 Botany S Agriculture > SB Plant culture > SB1-1110 Plant culture
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	26 Jun 2024 14:40
Last Modified:	26 Jun 2024 14:40
URI:	https://eprints.ums.edu.my/id/eprint/38943

Actions (login required)

View Item