Yap, Philip Yao Kong (2014) Phytochemicals, antibacterial, antioxidants and acetylcholinesterase inhibition properties of selected honey of Sabah. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Honey is a natural product that is produced by honey bee from floral nectar, transformed and stored in the honeycomb to mature. Honey contained various phytochemicals (i.e phenolic and flavonoid contents) compounds which could contribute to human health. The objectives of this research were to determine the physicochemical and phytochemical properties of honey and to test the effectiveness of honey extracts as antibacterial, antioxidant and anti-Alzheimer’s agent. Ten honey of Sabah were chosen which consist of four wild honey [Apis cerana (collected in Tenom), Apis andreniformis (collected in Tenom), Apis nuluensis (collected in Ranau), Apis koschevnikovi (collected in Tenom)] and six commercial honey (young and old Mangrove, young and old Upper Mountain, Tropical, Potiukan) which were supplied by the local stores. Manuka honey and sugar analogue were used as positive and negative controls. All samples were extracted using 80% and absolute methanol. pH, free acidity, conductivity, moisture and dry content were determined based on the Official Method of Analysis and absorbance of colour intensity using spectrophotometric method. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau and aluminium chloride calorimetric methods. Antibacterial activities were determined using disc diffusion and broth dilution methods. Antioxidant activities were determined using Ferric Reducing Antioxidant Power (FRAP) assay, DPPH (2,2- diphenyl-1-pycrylhydrazyl) method and ABTS decolorization assays. Acetylcholinesterase activity was determined using Ellman method. Results for physicochemical and phytochemical test showed that honey produced by wild A.cerana contained the highest colour intensities, conductivity, total phenolic and flavonoid contents with the values of 0.39 ± 0.08, 0.72 ± 0.01 mS/cm, 10.43 ± 0.02 mg GAE/ g and 8.76 ± 0.00 mg RE/g in 80% methanol extract. Honey produced by wild A.cerana showed maximum inhibition against Bacillus cereus with the value of 16.00 ± 0.33 mm in 80% methanol extract. Honey produced by wild A.cerana also displayed the highest antiradical (DPPH) and ABTS scavenging activity with the values of 83.68 ± 0.36% and 8.04 ± 0.06 mg AEAC/g respectively (at 100 μg/ml), while wild A.nuluensis honey extract was highest in FRAP assay with the value of 43.87 ± 0.04 μM/g (in 80% methanol extract). Acetylcholinesterase inhibition was strongest displayed by wild A.nulunesis honey extract with the value of 75.21 ± 0.21% (at 100 μg/ml of 80% methanol extract). In conclusion, selected honey of Sabah contained a wide range of phenolic and flavonoid compounds which contributes to the biological activities which possessed diverse potential health benefits.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Honey, Apis cerana, Apis andreniformis, Apis nuluensis, Apis koschevnikovi, Wild honey, Commercial honey |
| Subjects: | Q Science > QK Botany > QK1-989 Botany > QK710-899 Plant physiology |
| Department: | INSTITUTE > Institute for Tropical Biology and Conservation |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 11 Mar 2025 10:02 |
| Last Modified: | 11 Mar 2025 10:02 |
| URI: | https://eprints.ums.edu.my/id/eprint/43094 |
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