Chong, Yen Thing (2013) Characterization of Photobacterium sp. and development of vaccine against vibriosis in Asian seabass, Lates calcarifer. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Asian seabass is recognised as one of the most important aquaculture fish species in Sabah. Tg. Badak hatchery is one of the most prominent hatcheries in the area of Kota Kinabalu, Sabah as the supplier of the Asian seabass seed. An unfortunate incident which caused mortality of the Asian seabass juveniles had occurred in Tg. Badak hatchery. The dead and moribund fish experienced abdominal swelling, hemorrhage, skin lesion, and exophthalmia. The affected fish were observed died within 2 to 3 days after the onset of the disease. Biochemical and molecular characterization of the isolated bacteria from internal organs of the dead and moribund fish showed the presence of pathogenic bacterium, Photobacterium damselae in a significant number. Bacterial disease is a common factor affecting aquaculture industry worldwide. Vibriosis is found as one of the fatal diseases affecting Asian seabass in Sabah. In view of the problem, vaccination is seen as the only hope for future prevention of the disease. Thus, this study was conducted with the aim to formulate a suitable vaccine, along side with the best way and right time for booster vaccination in Asian seabass, Lates calcarifer against vibriosis. Asian seabass was tested for its immunological competency by performing agglutination tests with its sera collected during the vaccination period. Agglutination started on the third day and lasted until the ninth week post vaccination. In this study, vaccination was demonstrated through injection with and without adjuvant, and hyperosmotic-pretreatment immersion. In the vaccination experiment, test fish were divided into 4 groups consisting of control, non-booster, 1-booster and 2-booster groups. At the end of experiment, all fish were challenged by intraperitoneal injection with live pathogenic strain of Vibrio harveyi (VHJR7). The injection groups were tested at LD60, LD80 and LD100 whereas the immersion group was tested at LD50 and LD100. The number of mortality in each group was recorded. The efficiency of vaccination was expressed in relative percentage of survival (RPS). High RPS (>80%) recorded in all injected groups of fish challenged with LD60. Whereas high RPS (>80%) recorded in the hyperosmotic-pretreatment immersion with twice booster. On one hand, the vaccination using killed antigen has been shown to protect Asian seabass from vibriosis but on the other hand, the discovery of another bacterial pathogen, Photobacterium damselae requires great attention to be paid upon the study of distribution, severity of economic impacts and prevention of the bacterial pathogen in aquaculture of marine fish in Sabah.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Asian seabass, Lates calcarifer, Vibriosis, Photobacterium damselae, Vaccination, Immunological competency, Agglutination test |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture > SH151-179 Fish culture > SH171-179 Diseases and adverse factors |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 20 Mar 2025 10:24 |
| Last Modified: | 20 Mar 2025 10:24 |
| URI: | https://eprints.ums.edu.my/id/eprint/43271 |
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