Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: a mini-review

Ammar Akram Kamarudin and Mohamad Zulhafiz Shafiq Zulhilmi Cheng and Norhaizan Mohd. Esa (2024) Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: a mini-review. Transactions on Science and Technology, 11 (4-2). pp. 1-10.

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Abstract

In vitro cell culture models are crucial tools in drug discovery for understanding pathophysiology and cellular physiology. Traditionally, two-dimensional (2D) cultures have significantly advanced cell-based research. However, their translational potential is limited by their inability to replicate the natural cellular environment. The emergence of threedimensional (3D) culture models offers more physiological relevance platforms to mimic a natural tissue architecture. Scaffold-based techniques that utilize extracellular matrix (ECM) components have become increasingly prominent, with collagen emerging as a leading candidate due to its structural and biological properties. Collagen, the key player of structural protein in connective tissue, may act as biomimetic scaffolds that facilitate cell aggregation, proliferation, and migration. This mini-review explores the unique features of collagen as a hydrogel scaffold, including its biocompatibility, natural abundance, and the capacity to replicate tissue-specific architectures.

Item Type:	Article
Keyword:	2D and 3D cultures, In vitro, Scaffolds, Collagen, ECM
Subjects:	Q Science > Q Science (General) > Q1-390 Science (General) > Q1-295 General T Technology > TX Home economics > TX1-1110 Home economics > TX341-641 Nutrition. Foods and food supply
Department:	FACULTY > Faculty of Food Science and Nutrition
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	02 May 2025 10:59
Last Modified:	02 May 2025 10:59
URI:	https://eprints.ums.edu.my/id/eprint/43694

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