Isabella Ebi and Yong, Annita Seok Kian and Lim, Leong Seng and Lin, Yu-Hung and Rossita Shapawi (2023) Dietary α‑tocopherol acetate improved growth performance, lipid oxidative status, and disease resistance of hybrid grouper (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂). Fisheries Science, 89. pp. 477-485. ISSN 0919-9268
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Abstract
The present study aimed to evaluate the effects of dietary α-tocopherol on growth performance, lipid oxidative status, and disease resistance against Vibrio harveyi in hybrid grouper (Epinephelus fuscoguttatus × E. lanceolatus). The experimental fish (7.74 ± 0.14 g) were fed diets with different α-tocopherol levels of 10.1 mg/kg (E10; control diet), 121.5 mg/kg (E122), 231.6 mg/kg (E232), 416.3 mg/kg (E416), and 815.4 mg/kg (E815) for 10 weeks. At the end of the feeding trial, the α-tocopherol levels significantly influenced growth performance, with fish fed E122 having significantly higher weight gain and specific growth rate than fish fed E10 (p < 0.05). Supplementation of α-tocopherol increased the total feed intake (FI) in hybrid grouper, with fish fed E815 showing significantly higher total FI than fish fed E232, however, no significant effects were observed in both FCR and FE parameters among fish treatments. Fish in all treatments demonstrated high survival, indicating that hybrid grouper was tolerant to short-term feeding with a deficient-α-tocopherol diet. Muscle and hepatic α-tocopherol contents increased with the increase in α-tocopherol levels. Meanwhile, the whole-body proximate composition, hematological parameters, and biochemical parameters were not affected by the α-tocopherol levels. The hepatic thiobarbituric acid reactive substances gradually decreased as the α-tocopherol inclusion increased, indicating the important role of α-tocopherol as an antioxidant. The findings also show that α-tocopherol supplementation improved the fish disease resistance against V. harveyi. Although α-tocopherol supplementation at 122 mg/kg diet can support growth and protect fish from bacterial infection, a higher level of E415 or 815 mg/kg provided the best protection from lipid oxidation.
| Item Type: | Article |
|---|---|
| Keyword: | α-Tocopherol, Hybrid grouper, Growth, Lipid oxidation, Hematological parameters, Disease resistance |
| Subjects: | Q Science > QP Physiology > QP1-(981) Physiology > QP501-801 Animal biochemistry S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture > SH151-179 Fish culture |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 03 Jul 2025 15:40 |
| Last Modified: | 03 Jul 2025 15:40 |
| URI: | https://eprints.ums.edu.my/id/eprint/44334 |
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