Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats

Muhammad Dawood Shah, and Mohammad Iqbal, and Charles Gnanaraj, and ATM Emdadul Haque, (2015) Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats. Pharmaceutical Biology, 53 (1). pp. 31-39. ISSN 13880209

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Abstract

Context: Nephrolepis biserrata L. (Nephrolepidaceae) has been used in folk medicine for protection against different diseases. Objective: The current research investigated the protective effect of the methanol extract of N. biserrata leaves against carbon tetrachloride (CCl4)-induced hepatic damage in rats. Materials and methods: Total phenolic content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were estimated. In addition, Sprague-Dawley (SD) rats were randomly divided into six groups: control, CCl4 (1.0 mg/kg b wt), N. biserrata extract (at doses of 125, 250, and 375 mg/kg b wt) with CCl4 and N. biserrata extract (at dose of 375 mg/kg b wt) alone. After 2 weeks all rats were sacrificed and hepatoprotective effect of N. biserrata was evaluated. Results: Our results indicated that the high total phenolic content (127.28 ± 1.57 mg GAE/g) of N. biserrata may be the major contributor to strong antioxidant activities. Moreover, N. biserrata significantly depleted the elevation of enzymatic levels of alanine aminotransferase and aspartate aminotransferase (20-93% recovery), reduced the extent of malondialdehyde (47-90% recovery), increased the level of reduced glutathione (25-39% recovery), and elevated the activities of catalase, glutathione reductase, glutathione peroxidase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and quinone reductase (5-34% recovery). Histopathological observations also revealed that N. biserrata decreased fatty degeneration and necrosis in CCl4 administered rats. Discussion and conclusion: N. biserrata has strong antioxidant activities and significant protective effects against CCl4 induced hepatotoxicity in rats. © 2015 Informa Healthcare USA, Inc. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Antioxidant enzymes; Histopathology; Liver injury; Methanol extract; Oxidative stress; Total phenolic content
Subjects: Q Science > QD Chemistry
Divisions: SCHOOL > School of Education and Social Development
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 19 Jun 2015 02:29
Last Modified: 12 Oct 2017 03:25
URI: http://eprints.ums.edu.my/id/eprint/10945

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