Quantification and enrichment of sinensetin in the leaves of Orthosiphon stamineus

Hossain, Amzad and Zhari Ismail, (2012) Quantification and enrichment of sinensetin in the leaves of Orthosiphon stamineus. Arabian Journal of Chemistry . ISSN 1878-5352

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Official URL: http://dx.doi.org/10.1016/j.arabjc.2012.02.016

Abstract

Sinensetin was identified in the leaves of Orthosiphon stamineus by thin-layer chromatography, GC coupled with mass spectrometry (MS), UV, IR and 1H-NMR spectroscopy. Enrichment of sinensetin by using different mixture solvent system and the quantification analysis by thin-layer chromatography-imaging densitometric method using as external standard method showed the highest concentration of 0.32% of sinensetin in the mixture solvent of acetone-water (70:30) when compared to methanol-water (1:1) of only 0.15% in the leaves of Orthosiphon stamineus. The TLC densitometer, although yields slightly higher values than the other analytical methods, is preferred due to its simplicity, ease and low cost.

Item Type:Article
Uncontrolled Keywords:Acetone-water, Densitometer, Enrichment, Mixture solvents, Sinensetin
Subjects:?? QD241-441 ??
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:1236
Deposited By:IR Admin
Deposited On:02 Jan 2013 11:17
Last Modified:23 Feb 2015 11:12

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