Crude proteins, total soluble proteins, total phenolic contents and SDS-PAGE profile of fifteen varieties of seaweed from Semporna, Sabah, Malaysia

Mohd Rosni Sulaiman, and Fisal Ahmad, and Patricia Anthony, and Chye, Fook Yee and Azwal Aziz @ Wilter Malandi, (2015) Crude proteins, total soluble proteins, total phenolic contents and SDS-PAGE profile of fifteen varieties of seaweed from Semporna, Sabah, Malaysia. International Food Research Journal , 22 (4). pp. 1483-1493. ISSN 1985-4668

[img]
Preview
PDF
49Kb

Official URL: http://www.ifrj.upm.edu.my/22%20(04)%202015/(24).p...

Abstract

It is crucial to determine several protein-related parameters at the initial stages of proteomic analysis of any biological samples. In this study, crude protein content, total soluble protein, total phenolic content and the SDS-PAGE profile of fifteen varieties of seaweed from Semporna, Sabah, Malaysia were analysed. The crude protein, total soluble protein and total phenolic content of all seaweed samples were in the range of 3.99 to 13.18 % of dry weight, 0.52 to 1.45 mg/mL in acetone dried powder samples and 8.59 to 48.98 mg PGE/g dry weight, respectively. In general, the differences (crude protein, total soluble protein and total phenolic content) among all fifteen varieties of seaweeds were significant (p<0.05). There was also a strong positive correlation between crude protein and total soluble protein concentration (Pearson’s Correlation Coefficient (r)=0.923; p=0.01) in these fifteen varieties of seaweed. A distinctive protein pattern was observed in the SDS-PAGE gels between three different seaweed classes of green, red and brown colours. All of these results are important in sample preparations (extractions) before furthering proteomic analysis in order to identify and characterize seaweed proteomes.

Item Type:Article
Uncontrolled Keywords:Seaweed, Protein, Phenolic, Phenol extraction, Proteome
Subjects:T Technology > TX Home economics > Nutrition. Foods and food supply
Divisions:FACULTY > Faculty of Food Science and Nutrition
ID Code:12535
Deposited By:IR Admin
Deposited On:11 May 2016 14:08
Last Modified:11 May 2016 14:08

Repository Staff Only: item control page


Browse Repository
Collection
   Articles
   Book
   Speeches
   Thesis
   UMS News
Search
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository