Sintesis enzimatis flavonoid-glikosida dari gambir (Uncaria gambir) menggunakan enzim CGT-ase dari bacillus licheniformis

Joko Sulistyo, and Norman Ferdinal, and Novizar Nazir, (2013) Sintesis enzimatis flavonoid-glikosida dari gambir (Uncaria gambir) menggunakan enzim CGT-ase dari bacillus licheniformis. In: Prosiding Semirata FMIPA Universitas Lampung, 2013, 10-12 May 2013, Universitas Lampung, Indonesia.

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Abstract

Laboratory scale-up of Flavonoid-�- glycoside was synthesized using enzyme of CGT-ase which was isolated from cultivated of Bacillus Licheniformis. CGT-ase enzyme has optimum capability at the temperature of 45C, pH 6.5. yielded 7.864 unit/minute/mL. The pretest of CGT-ase transfer activity was carried out using pyrocatechol and resorcinol as an acceptor and iso-malto and commercial starch solution as the glucosyl donor. Subsequently, acceptor was replaced by flavonoid (Etyl acetate and butanol fraction) extracted from four types of gambir from West Sumatra.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Uncontrolled Keywords: Uncaria gambir, Bacillus Licheniformis, flavonoid--glicoside
Subjects: T Technology > TP Chemical technology
Divisions: FACULTY > Faculty of Food Science and Nutrition
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 07 Jan 2016 06:37
Last Modified: 10 Nov 2017 02:19
URI: http://eprints.ums.edu.my/id/eprint/12654

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