Isolation and characterization of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in soils from sarawak

Mohd Khalizan Sabullah, and Siti Aqlima Ahmad, and Muhammad Fawwaz Shukor, and M.F. Rahman, and M.S. Shukor, and Nor Aripin Shamaan, and Muhammad Fawwaz Shukor, (2016) Isolation and characterization of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in soils from sarawak. Agrivita, 38 (1). pp. 1-13. ISSN 1260537

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Abstract

Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. © 2016, Agriculture Faculty Brawijaya University.

Item Type: Article
Uncontrolled Keywords: bioreduction; glyphosate; Klebsiella oxytoca; molybdenum blue; molybdenum-reducing bacterium
Subjects: T Technology > TD Environmental technology. Sanitary engineering
Divisions: FACULTY > Faculty of Food Science and Nutrition
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 04 Mar 2016 02:52
Last Modified: 16 Oct 2017 07:35
URI: http://eprints.ums.edu.my/id/eprint/12928

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