Polymerase chain reaction-restriction fragment length polymorphism method for differentiation of uropathogenic specific protein gene types

Yun, Mei Lai and Zaw, Lin and Shamsul Bahari Shamsudin, and Myo Thura Zaw, (2016) Polymerase chain reaction-restriction fragment length polymorphism method for differentiation of uropathogenic specific protein gene types. Journal of Microbiology, Immunology and Infection, 49 (4). pp. 591-594. ISSN 1684-1182

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Abstract

The putative pathogenicity island (PAI) containing the uropathogenic specific protein (usp) gene and three small open reading frames (orfU1, orfU2, and orfU3) encoding 98, 97, and 96 amino acid proteins is widely distributed among uropathogenic Escherichia coli (UPEC) strains. This PAI was designated as PAIusp. Sequencing analysis of PAIusp has revealed that the usp gene can be divided into two types - uspI and uspII - based on sequence variation at the 3' terminal region and the number and position of orfUs differ from strain to strain. Based on usp gene types and orfU sequential patterns, PAIusp can be divided into four subtypes. Subtyping of PAIusp is a useful method to characterize UPEC strains. In this study, we developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to differentiate usp gene types. This method could correctly identify the usp gene type in usp-positive UPEC strains in our laboratory.

Item Type: Article
Uncontrolled Keywords: Pathogenicity island; Restriction fragment length polymorphism, Urinary tract infection, Uropathogenic Escherichia coli, Uropathogenic specific protein
Subjects: R Medicine > RB Pathology
Divisions: FACULTY > Faculty of Medicine and Health Sciences
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 26 Oct 2016 06:05
Last Modified: 23 Oct 2017 07:11
URI: http://eprints.ums.edu.my/id/eprint/14796

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