Detection of viable bacteria in environmental water samples using DNase I and PCR method. International

Teoh, Peik Lin and Ho, C. F. and Vun, Leong Wan (2016) Detection of viable bacteria in environmental water samples using DNase I and PCR method. International. International Journal of Environmental Studies, 73 (5). pp. 683-691. ISSN 1029-0400

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Official URL: http://dx.doi.org/10.1080/00207233.2016.1150093

Abstract

In this study, we tested the potential application of a previously developed method in detecting Escherichia coli in environmental water samples. To increase the sensitivity of the method, and the recovery of microbial cells, water samples were filtered before being subjected to DNase treatment and polymerase chain reaction amplification. Results showed that DNase I treatment and PCR reaction were not affected by inhibitors as the expected amplicon was successfully amplified in autoclaved environmental waters spiked with E. coli. Then, we applied this method to naturally contaminated environmental water samples. We firstly confirmed the presence of coliforms and E. coli in these water samples by plating in eosin methylene blue agar. Simultaneous PCR amplification targeting Lac Z and uidR gene of total coliforms and E. coli respectively demonstrated that this developed method is potentially applicable for routine microbial assessment of health risks related to viable microorganisms in environmental or drinking waters.

Item Type:Article
Uncontrolled Keywords:PCR, Viable, Heat kill, DNase, Environmental water
Subjects:G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:15138
Deposited By:IR Admin
Deposited On:13 Dec 2016 16:26
Last Modified:13 Dec 2016 16:26

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