Physical and stability characteristics of Burkholderia cepacia lipase encapsulated in κ-carrageenan

Kenthorai, Raman Jegannathan and Chan, Eng Seng and Pogaku, Ravindra (2009) Physical and stability characteristics of Burkholderia cepacia lipase encapsulated in κ-carrageenan. Journal of Molecular Catalysis B: Enzymatic, 58 (1-4). pp. 78-83. ISSN 1381-1177

[img]
Preview
PDF
207Kb

Official URL: http://dx.doi.org/10.1016/j.molcatb.2008.11.009

Abstract

In this work, a novel approach for lipase immobilization was exploited. Lipase from Burkholderia cepacia was encapsulated into κ-carrageenan by co-extrusion method to form a liquid core capsule. The diameter of the encapsulated lipase was found to be in the range of 1.3-1.8 mm with an average membrane thickness of 200 μm and 5% coefficient of variance. The encapsulation efficiency was 42.6% and 97% moisture content respectively. The encapsulated lipase was stable between pH 6 and 9 and temperature until 50 °C. The encapsulated lipase was stable until disintegration of the carrier when stored at 27 °C and retained 72.3% of its original activity after 6 cycles of hydrolysis of p-NPP. The encapsulated lipase was stable in various organic solvents including methanol, ethanol, iso-propanol, n-hexane and n-heptane. Kinetic parameters Km and Vmax were found to be 0.22 mM and 0.06 μmol/min for free lipase and 0.25 mM and 0.05 μmol/min for encapsulated lipase respectively. © 2008 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:κ-Carrageenan, Burkholderia cepacia lipase, Co-extrusion, Encapsulation, Immobilization, Liquid core capsule, Stability
Subjects:T Technology > TP Chemical technology
Divisions:SCHOOL > School of Engineering and Information Technology
ID Code:1576
Deposited By:IR Admin
Deposited On:16 Mar 2011 11:27
Last Modified:24 Feb 2015 11:06

Repository Staff Only: item control page


Browse Repository
Collection
   Articles
   Book
   Speeches
   Thesis
   UMS News
Search
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository