Drought effect on proteome pattern of leaves of Tr-8 rice variety

Kong, Choi Yan (2015) Drought effect on proteome pattern of leaves of Tr-8 rice variety. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Rice (Oryza sativa L.) is the most important food crops which provide a staple diet for almost half of the world's population. However, drought stress resulted from global climatic change severely limits the productivity of lowland irrigated rice. Proteins that are responsible for drought stress will express differently during drought which might be contributed to drought resistant mechanism and could led to the development of drought resistant rice by genetic engineering or marker-assisted selection breeding. In this study, proteome pattern of TR-8 variety rice between well-watered condition and drought stress condition was analyzed and compared. Four-week-old rice seedlings were exposed to drought condition for three, six, nine, twelve, and fifteen days. The proteins were extracted from leaves, separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and stained with silver nitrate solution. The relative water contents (RWC) under well-watered condition for fifteens days were similar and consistent, however, the relative water content under drought stress condition declined dramatically after six days drought stress. The rice seedlings reached their permanent wilting point after 15 days of drought stress. Qualitative variation on the protein expression by three and five protein bands were observed between well-watered and drought-stressed rice seedlings respectively. Protein bands under twelve days drought stress showed the most qualitative variation. Twodimensional electrophoresis and staining with Coomassie Briiliant Blue can be conducted for future improvement.

Item Type:Academic Exercise
Uncontrolled Keywords:Rice (Oryza sativa L.),genetic engineering , marker-assisted selection breeding, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)
Subjects:S Agriculture > SB Plant culture
Divisions:FACULTY > Faculty of Sustainable Agriculture
ID Code:17079
Deposited By:Noraini L
Deposited On:14 Jun 2017 12:41
Last Modified:11 Jul 2017 08:02

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