Analysis of proteomic pattern of Aumentary Canal between larvae and adult moth of Conopomorpha Cramerella Snellen

Nur Emauna Zolkopli, (2015) Analysis of proteomic pattern of Aumentary Canal between larvae and adult moth of Conopomorpha Cramerella Snellen. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Cocoa Pod Borer is the most serious pest in Southeast Asia. Despite the damages done by the CPB, there is only few proceedings research done in order to cope with this problem. Samples of adult moth and larvae of CPB were collected from the Quoin Hill Research Center, Tawau, Sabah. The alimentary canals were extracted and mixed with lysis buffer (2.5% 50s, 5% (3-mercaptoethanol, and 62.5 mM Tris-HCI, pH 6.8). Differences of the proteins expressions between the alimentary canals of adult moth and larvae of CPB were investigated in effort to compare and determine the protein bands that vary quantitatively and qualitatively between both the adult moth and larvae. The protein bands were separated by using 12.5% SDS-PAGE which were stained with Coomassie Brilliant Blue (CBB) and silver staining. The protein bands were observed and analyzed with Bio-Rad Gel Imager and Image Lab software version 4.1 by Bio-Rad at default 70% sensitivity. From the SDS-PAGE, there were various differences between adult moth and larvae in terms of their protein expression. The adult moth consisted more of low molecular weight protein bands ranging from 50 -80 kDa compared to the larvae which have more protein bands that were high in their molecular weight ranging 80 - 250 kDa. From this research, there could be a possibility that the proteins and enzymes expressed were used in the food digestion of the CPB. Other than that, the proteins expressed could be involved in the restructuring of the guts of the CPB larvae to the adult moth.

Item Type: Academic Exercise
Uncontrolled Keywords: Cocoa Pod Borer, Coomassie Brilliant Blue (CBB) , CPB larvae
Subjects: S Agriculture > S Agriculture (General)
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: MDM FAUZIAH MATSIN
Date Deposited: 05 Jul 2017 01:59
Last Modified: 13 Oct 2017 07:54
URI: http://eprints.ums.edu.my/id/eprint/17104

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