The application of the brine shrimp arteml4 salina for delivery of biologicalm oleculesin aquaculture

Nur Idayu Adnan, (2014) The application of the brine shrimp arteml4 salina for delivery of biologicalm oleculesin aquaculture. Universiti Malaysia Sabah. (Unpublished)

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Abstract

"The brine shrimp Anemia saiina (Linn, 1758) is generally used as live feed in the aquaculture industry. The relative ease of propagation, tolerance to extreme salinity and high protein content make Artemia sauna ideal for feeding marine organisms at the post-larval stage. The ability of Artemia to feed on microbes makes them amenable to application for the delivery of recombinant protein molecules and recombinant DNA into marine organisms. This investigation focuses on the application of the Artemia for the delivery of recombinant protein produced in Escherichiac oil (BL21) using a Green FluorescentP rotein (GFP)r eporter as well as a recombinantp lasmid PMCS2.1-ENTH-GFwPh ich contains eukaryotic promoter and terminator elements. The findings of this study will facilitate our understanding of the process by which Artemia can uptake recombinant proteins and express plasmid DNA constructs containing recombinant protein coding genes. From this study, the Artemia has been able to retain the GFP produced by the Esc/herichiac oil (BL21) which make it suitable for the protein retention and delivery to aquaculture organisms but the Artemia unable to transcribe the gene from plasmid PMCS2.1- ENTH-GFPw hich has been fed orally. However, the Artemia could express the protein from plasmid PMCS2.1-ENTH-GFPu sing biolistic gun method in transformation. Therefore, from this experiment, it can be conclude that Artemia besides having unique characteristics, it can be manipulated for the delivery of biological molecules in aquaculture.

Item Type: Academic Exercise
Uncontrolled Keywords: Aquaculture, fish, seafood product
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Noraini
Date Deposited: 27 Nov 2017 02:01
Last Modified: 27 Nov 2017 02:01
URI: http://eprints.ums.edu.my/id/eprint/17665

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