An alternative bioassay using Anabas testudineus (Climbing perch) cholinesterase for metal ions detection

Siti Aqlima Ahmad, and Wong, Y. F and Mohd Yunus Shukor, and Mohd Khalizan Sabullah, and Nur Adeela Yasid, and N. M Hayat, and Nor Aripin Shamaan, and Ariff Khalid, and Mohd. Arif Syed, (2016) An alternative bioassay using Anabas testudineus (Climbing perch) cholinesterase for metal ions detection. International Food Research Journal, 23 (4). pp. 1446-1452. ISSN 1985-4668

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Abstract

Climbing Perch or its scientific name, Anabas testudineus is one of the freshwater fish belonging to the family of Anabantidae. It is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exchange chromatography. This purification method provided a recovery yield of 5.36% with a purification fold of 6.6. The optimum conditions for ChE assay were identified to be 2.5 mM of butyrylthiocholine iodide (BTC) with pH 8.0 in Tris-HCl buffer at 40°C. Substrate specificity profile also indicated that ChE favours BTC as substrate because it records the highest catalytic efficiency (V/K). Protein analysis through Native-PAGE showed that ion exchange chromatography is an effective method to partially purify ChE. Metal ion inhibition tests were conducted and mercury (Hg) was found to show the highest inhibition effect (87.30%) whereas lead (Pb) shows the lowest inhibition effect (28.01%). All these findings showed that partially purified ChE from the liver of A. testudineus is suitable to be used as a bioindicator to detect the presence of metal ions.

Item Type: Article
Uncontrolled Keywords: Bioindicator,Cholinesterase (ChE),Inhibition,Metal ions
Subjects: T Technology > TP Chemical technology
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Munira
Date Deposited: 18 Feb 2018 11:57
Last Modified: 18 Feb 2018 11:57
URI: http://eprints.ums.edu.my/id/eprint/18842

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