Growth performance, soil chemical properties and Proteome analysis of Tr-8 Paddy variety under different application rate of Chitosan And NPK fertiuzer in Silabukan Soil

Woo, Mun Kit (2016) Growth performance, soil chemical properties and Proteome analysis of Tr-8 Paddy variety under different application rate of Chitosan And NPK fertiuzer in Silabukan Soil. Universiti Malaysia Sabah. (Unpublished)

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Abstract

A study on the effect of different application rate of chitosan and NPK to the growth performance and proteome pattern of TR-8 paddy variety was conducted. NPK fertilizer was applied at (0 kg N ha-1, 0 kg P20 S ha-1,0 kg K20 ha-1 ), (90 kg N ha-t, 60 kg P20 S ha-l, 70 kg K20 ha-1), (130 kg N ha-t, 70 kg P20 S ha-1 , 110 kg K20 ha-1) and (170 kg N ha-l, 80 kg P20 S ha-1 , 150 kg K20 ha-1) in 3 split applications. Chitosan was applied in the soil at 0%, 0.1%, 0.2% and 0.3% (w/w) of soil respectively. Agronomic parameters and soil samples were analyzed at harvesting stage while proteome analysis was conducted using sodium dodecyl sulfate polyacrylamide gel electrophoresis (50S-PAGE) on 2 different root samples at the age of 90 days. The results showed that chitosan and NPK fertilizer did not show any interaction on the vegetative growth and yield components in paddy and also soil chemical properties. The effect of chitosan alone did not contribute to the growth and yield of paddy. NPK fertilizer, on the other hand, did affect the growth and yield significantly when applied to those without fertilization. The soil nitrogen and soil pH was not affected by NPK fertilizer while soil available phosphorus was significantly affected by NPK fertilizer. This showed that NPK fertilizer played an important role on the growth performance in paddy. Based on SDS-PAGE analysis, there were total of 14 protein bands detected. Two of the bands showed two-folds in protein expression in roots with and without treatments. The expression could be plant response to nutrient stress and defense mechanisms. Overall, fertilization at 170 kg N ha-1 ,80 kg P20 S ha-1 and 150 kg K20 ha-1 gave the best growth performance. However, application at 130 kg N ha-1, 70 kg P20 S ha-1 and 110 kg K20 ha-1 could have similar effect and this could help farmers to reduce input cost on fertilizer.

Item Type: Academic Exercise
Uncontrolled Keywords: Paddy , fertilizer , soil
Subjects: S Agriculture > SB Plant culture
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Noraini
Date Deposited: 02 Mar 2018 02:31
Last Modified: 02 Mar 2018 02:31
URI: http://eprints.ums.edu.my/id/eprint/19062

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