Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)

Noor Aini Bohari, and Shafiquzzaman Siddiquee, and Kamaruzaman Hj. Ampon, (2016) Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol). Biosensors, 6 (3). p. 32. ISSN 2079-6374

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Abstract

Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. A formaldehyde biosensor was successfully developed by depositing an ionic liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), gold nanoparticles (AuNPs), and chitosan (CHIT), onto a glassy carbon electrode (GCE). The developed formaldehyde biosensor was analyzed for sensitivity, reproducibility, storage stability, and detection limits. Methylene blue was used as a redox indicator for increasing the electron transfer in the electrochemical cell. The developed biosensor measured the NADH electron from the NAD+ reduction at a potential of 0.4 V. Under optimal conditions, the differential pulse voltammetry (DPV) method detected a wider linear range of formaldehyde concentrations from 0.01 to 10 ppm within 5 s, with a detection limit of 0.1 ppm. The proposed method was successfully detected with the presence of formalin in fish samples, Lutjanus malabaricus and Thunnus Tonggol. The proposed method is a simple, rapid, and highly accurate, compared to the existing technique.

Item Type: Article
Uncontrolled Keywords: formaldehyde biosensor, glassy carbon electrode, gold nanoparticles, ionic liquid, methylene blue
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Munira
Date Deposited: 13 Mar 2018 03:24
Last Modified: 13 Mar 2018 03:24
URI: http://eprints.ums.edu.my/id/eprint/19364

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