Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™

Goh, Xiang Tong and Lim, Yvonne Ai Lian and Indra Vythilingam, and Chew, Ching Hoong and Lee, Ping Chin and Ngui, Romano and Tan, Tian Chye and Yap, Nan Jiun and Veeranoot Nissapatorn, and Chua, Kek Heng (2013) Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™. Malaria Journal, 12. p. 264. ISSN 14752875

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Background Plasmodium knowlesi is a simian malaria parasite that is widespread in humans in Malaysian Borneo. However, little is known about the incidence and distribution of this parasite in the Sandakan division, Malaysian Borneo. Therefore, the aim of the present epidemiological study was to investigate the incidence and distribution of P. knowlesi as well as other Plasmodium species in this division based on a most recent developed hexaplex PCR system (PlasmoNex™). Methods A total of 189 whole blood samples were collected from Telupid Health Clinic, Sabah, Malaysia, from 2008 to 2011. All patients who participated in the study were microscopically malaria positive before recruitment. Complete demographic details and haematological profiles were obtained from 85 patients (13 females and 72 males). Identification of Plasmodium species was conducted using PlasmoNex™ targeting the 18S ssu rRNA gene. Results A total of 178 samples were positive for Plasmodium species by using PlasmoNex™. Plasmodium falciparum was identified in 68 samples (38.2%) followed by 64 cases (36.0%) of Plasmodium vivax, 42 (23.6%) cases of P. knowlesi, two (1.1%) cases of Plasmodium malariae and two (1.1%) mixed-species infections (i e, P. vivax/P. falciparum). Thirty-five PlasmoNex™ positive P. knowlesi samples were misdiagnosed as P. malariae by microscopy. Plasmodium knowlesi was detected in all four districts of Sandakan division with the highest incidence in the Kinabatangan district. Thrombocytopaenia and anaemia showed to be the most frequent malaria-associated haematological complications in this study.

Item Type: Article
Uncontrolled Keywords: Plasmodium knowlesi, malaria parasite, PlasmoNex™
Subjects: R Medicine > RC Internal medicine
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Munira
Date Deposited: 07 Aug 2018 07:13
Last Modified: 07 Aug 2018 07:13

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