Mariam Abd Latip, Datin and Rosmah Murdad, and Zaleha Abdul Aziz, and Ting, Lai Hui and Lilly Malar, Govindasamy and Rimi Ripin, (2010) Effects of N6-benzyladenine and thidiazuron on proliferation of phalaenopsis gigantea protocorms. Asia-Pacific Journal of Molecular Biology and Biotechnology, Volume 18, Issue 1, 2010, Pages 215-218 (1). pp. 215-218. ISSN 0128-7451
Official URL: http://www.scopus.com/record/display.url?view=basi...
Cytokinins are known to promote multiplication and development of protocorms in orchid plants. Potential effects of N6-benzyladenine (BA) and thidiazuron (TDZ) on the proliferation of Phalaenopsis gigantea protocorms were investigated. The seed-derived protocorm sections were cultured on solidified NDM (New Dogashima Medium) basal medium containing 0-5.0 mg l-1 BA; or 0-1.0 mg l-1 TDZ. Sections cultured on the media containing low concentrations of BA (0.5 to 3.5 mg l-1) or TDZ (0.1 to 1.0 mg l-1) multiplied faster as compared to those cultured on other media with higher concentrations of BA and the control medium. Approximately 28% of the sections proliferated after 40 days in culture. The percentage of protocorm sections proliferate increased significantly up to 58 -66% (with 1-20 new protocorms per section) on media containing TDZ at the concentrations of 0.1 - 0.3 mg l-1 after 80 days of culture. In the presence of BA, however, the percentage of sections produced new protocorms was significantly lower, ranging from 5 to 30% with 1-46 new protocorms formed per section.
|Uncontrolled Keywords:||Orchid, Phalaenopsis gigantea, Propagation, Protocorms|
|Subjects:||?? QK495 ??|
|Divisions:||SCHOOL > School of Sustainable Agriculture|
|Deposited By:||IR Admin|
|Deposited On:||10 Mar 2011 18:32|
|Last Modified:||18 Feb 2015 11:31|
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