Enzymatic synthesis of polyphenol glycosides catalyzed by transglycosylation reaction of cyclodextrin glucanotransferase derived from Trichoderma viride

Sohaib Nazir, and Joko Sulistyo, and Muhammad Iqbal Hashmi, and Ai, Ling Ho and Mohammad Shaheen Khan, (2018) Enzymatic synthesis of polyphenol glycosides catalyzed by transglycosylation reaction of cyclodextrin glucanotransferase derived from Trichoderma viride. International Journal for Research in Applied Science & Engineering Technology, 55 (8). pp. 3026-3034.

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Abstract

Present study was conducted to evaluate the ability of Trichoderma viride as a source of cyclodextrin glucanotransferase that has shown transglycosylation activity in the presence of polyphenolic constituents extracted from Moringa oleifera leaves as its acceptor and wheat flour as its substrate to catalyze synthesis of polyphenolic glycosides as transglycosylation (transfer) reaction products. The enzymatic synthesized polyphenolic glycosides were then purified using octa-dodecyl-functionalized silica gel column chromatography prior to analysis using thin layer chromatography and high performance liquid chromatography and identified using nuclear magnetic resonance (NMR) spectroscopy. The high performance liquid chromatogram performed that the isolated transglycosylation products had retention times and concentration at 1.446 min (0.0017 mg/ml), 1.431 min (0.14 mg/ml), and 1.474 min (0.012 mg/ml), respectively, compared to the retention time of arbutin (1.474 min) that was applied as authentic standard for polyphenol glycoside. Moreover, observation using 1H NMR as well as 13C NMR showed that structures of the transglycosylation products were identified as gallic acid-4-O-β-glucopyranoside, ellagicacid-4-O-β-glucopyranoside, and catechin-4′-O-glucopyranoside, respectively.

Item Type: Article
Uncontrolled Keywords: Trichoderma viride, Cyclodextrin glucanotransferase, Transglycosylation reaction, Moringa oleifera, Polyphenolic glycoside
Subjects: Q Science > QK Botany
Divisions: FACULTY > Faculty of Food Science and Nutrition
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 15 Aug 2019 00:30
Last Modified: 15 Aug 2019 00:30
URI: http://eprints.ums.edu.my/id/eprint/23312

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