Effect of organic soil Amendments on growth, Phytochemical content and antimicrobial activity of Misai Kucing (Orthosiphon Stamineus)

Chee Hoyau, (2017) Effect of organic soil Amendments on growth, Phytochemical content and antimicrobial activity of Misai Kucing (Orthosiphon Stamineus). Universiti Malaysia Sabah. (Unpublished)

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Abstract

Extensive research has been carried out on Misai Kucing ( 0rthosiphon stamineus Benth) from the family of Lamiaceae since 1930s. It has been widely used as herbal tea called 'Java tea' in Asian and European countries with various types of pharmacological properties and medicinal values. An experiment was conducted at Faculty of Sustainable Agriculture in Universiti Malaysia Sabah to investigate the effect of organic soil amendments on growth and yield, phytochemical content, antioxidant and antimicrobial activity of 0rthosiphon stamineus. This is due to the lack of studies regarding the effect of plant-based organic soil amendments on secondary metabolites in 0. stamineus plant. The experiment was conducted under the rain shelter of the campus in polybags filled with topsoil, sand and organic matter at a ratio of 3:2:1, in which the organic matters were the treatments used. Plant samples were harvested after 12 weeks of planting with plant growth parameters measured weekly and the samples were extracted for secondary metabolites analysis. The experimental design used in this study was completely randomised design (CRD), using spent mushroom substrate (SMS), rice husk, empty fruit bunch (EFB), rice bran and cocopeat as organic soil amendment treatments with five replicates each. Data were analysed with one way ANOVA using SPSS software and the treatment means were compared by Tukey's test at 5% of significance level. Generally, 0rthosiphon stamineus plants treated with organic soil amendments showed significant difference in growth and yield over control medium. The best performance in growth and yield of 0. stamineus resulted from EFB amendment planting medium. Total phenolic content of 0. stamineus was the highest for planting medium treated with rice husk with value of 16.35 ± 2.86 mg GAE/g while total flavonoid content of plant was the highest in planting medium treated with spent mushroom substrate (SMS) with value of 13.43 ± 1.12 mg QE/g. Antioxidant activity of 0. stamineus was best performed in planting medium treated by rice husk, which showed ICso inhibition value of 5301.14 ± 389.38 µg/ml. For antimicrobial activity analysis, 0. stamineus was found to have inhibition against bacteria Escherichia coli and Bacillus cereus only without significant difference among treatments. The amendments of rice husk and SMS in planting medium has a potential to improve phytochemical content and antioxidant activity in 0. stamineus.

Item Type: Academic Exercise
Uncontrolled Keywords: herbal tea , herbs , Lamiaceae
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Depositing User: Noraini
Date Deposited: 31 Oct 2019 07:47
Last Modified: 31 Oct 2019 07:47
URI: http://eprints.ums.edu.my/id/eprint/23929

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